中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
7期
2957-2962
,共6页
李平%赵兵%吴德俊%王少华%李德坤%江涛
李平%趙兵%吳德俊%王少華%李德坤%江濤
리평%조병%오덕준%왕소화%리덕곤%강도
微RNAs%miR-181 b%胶质瘤干细胞%替莫唑胺%化疗耐受
微RNAs%miR-181 b%膠質瘤榦細胞%替莫唑胺%化療耐受
미RNAs%miR-181 b%효질류간세포%체막서알%화료내수
MicroRNAs%miR-181b%Glioma stem cells%Temozolomide%Chemotherapy tolerance
目的本研究旨在建立miR-181 b慢病毒载体感染U87胶质瘤干细胞模型后，检测其对TMZ化疗耐受性的变化，并探讨miR-181 b对于恶性脑胶质瘤（ GBM ）辅助化学治疗的意义。方法通过miR-181 b慢病毒表达载体转染U87胶质瘤干细胞，提高miR-181 b在U87胶质瘤干细胞中的表达。进一步通过RT-PCR、二次神经球形成实验、琼脂糖集落形成实验、MTT实验来分析miR-181 b对于 U87胶质瘤干细胞在TMZ化疗耐受性方面的作用。结果 miR-181 b慢病毒表达载体转染U87胶质瘤干细胞后，U87胶质瘤干细胞中的miR-181b明显上调，而且U87胶质瘤干细胞的生长受到明显抑制；miR-181b可以加强TMZ抑制U87胶质瘤干细胞的生长的作用。结论 miR-181b可以加强TMZ抑制U87胶质瘤干细胞的生长，miR-181b可能对于提高U87胶质瘤干细胞对TMZ的敏感性，降低化疗耐受性起到一定作用。
目的本研究旨在建立miR-181 b慢病毒載體感染U87膠質瘤榦細胞模型後，檢測其對TMZ化療耐受性的變化，併探討miR-181 b對于噁性腦膠質瘤（ GBM ）輔助化學治療的意義。方法通過miR-181 b慢病毒錶達載體轉染U87膠質瘤榦細胞，提高miR-181 b在U87膠質瘤榦細胞中的錶達。進一步通過RT-PCR、二次神經毬形成實驗、瓊脂糖集落形成實驗、MTT實驗來分析miR-181 b對于 U87膠質瘤榦細胞在TMZ化療耐受性方麵的作用。結果 miR-181 b慢病毒錶達載體轉染U87膠質瘤榦細胞後，U87膠質瘤榦細胞中的miR-181b明顯上調，而且U87膠質瘤榦細胞的生長受到明顯抑製；miR-181b可以加彊TMZ抑製U87膠質瘤榦細胞的生長的作用。結論 miR-181b可以加彊TMZ抑製U87膠質瘤榦細胞的生長，miR-181b可能對于提高U87膠質瘤榦細胞對TMZ的敏感性，降低化療耐受性起到一定作用。
목적본연구지재건립miR-181 b만병독재체감염U87효질류간세포모형후，검측기대TMZ화료내수성적변화，병탐토miR-181 b대우악성뇌효질류（ GBM ）보조화학치료적의의。방법통과miR-181 b만병독표체재체전염U87효질류간세포，제고miR-181 b재U87효질류간세포중적표체。진일보통과RT-PCR、이차신경구형성실험、경지당집락형성실험、MTT실험래분석miR-181 b대우 U87효질류간세포재TMZ화료내수성방면적작용。결과 miR-181 b만병독표체재체전염U87효질류간세포후，U87효질류간세포중적miR-181b명현상조，이차U87효질류간세포적생장수도명현억제；miR-181b가이가강TMZ억제U87효질류간세포적생장적작용。결론 miR-181b가이가강TMZ억제U87효질류간세포적생장，miR-181b가능대우제고U87효질류간세포대TMZ적민감성，강저화료내수성기도일정작용。
Objective To establish the miR-181 b lentiviral vector infected the U 87 glioma stem cells model,than detect the U87 glioma stem cells′chemotherapy tolerance to temozolomide (TMZ),and to explore the significance of the miR-181b for malignant glioma (gliomablastoma multiforme,GBM) in the adjuvant chemotherapy. Methods Using the miR-181 b lentiviral vector transfected the U 87 glioma stem cells and improve the level of miR-181b in the U87 glioma stem cells.RT-PCR,Secondary neurospheres formed assay ,agarose colony formation assay and MTT assay were used to detect the role of the miR-181b affect the U87 glioma stem cells in the TMZ chemotherapy tolerance .Results The level of miR-181b in the U87 glioma stem cells was significantly increased after miR-181b lentiviral vectors were transfected the U87 glioma stem cells,and the growth of U87 glioma stem cells were significantly inhibited.Furthermore,miR-181b can strengthen the role of TMZ in the growth inhibition of U87 glioma stem cells .Conclusions We found that miR-181 b can strengthen the role of TMZ in the growth inhibition of U87 glioma stem cells,miR-181b may play a certain role of improving the sensitivity of U 87 glioma stem cells to TMZ and reducing the chemotherapy tolerance .
