CAJ | 학술논문

目的研究羧胺三唑（ CAI）对小鼠Lewis肺癌移植瘤生长的抑制作用及其作用机制。初步探索CAI与小剂量地塞米松（DEX）合用是否有抑瘤增效作用。方法建立C57BL/6小鼠Lewis肺癌移植瘤模型，评估CAI、DEX及其合用组的抗肿瘤疗效。 ELISA法检测肿瘤匀浆中肿瘤坏死因子-α（ TNF-α）水平。免疫荧光法对提取的肿瘤内巨噬细胞进行鉴定。 RT-PCR法检测肿瘤内巨噬细胞中TNF-αmRNA表达水平。结果 CAI抑制移植瘤的生长。同时CAI能降低肿瘤内巨噬细胞中TNF-α的水平（ P＜0.01）。 CAI与小剂量DEX联合应用，其抑瘤作用及抑制TNF-α水平与单用组相比明显增强（ P＜0.05）。结论 CAI能够抑制移植瘤生长，其作用机制可能与抑制肿瘤中巨噬细胞的TNF-α水平有关。通过与地塞米松联合应用，可起到抗肿瘤增效作用。
목적연구최알삼서（ CAI）대소서Lewis폐암이식류생장적억제작용급기작용궤제。초보탐색CAI여소제량지새미송（DEX）합용시부유억류증효작용。방법건립C57BL/6소서Lewis폐암이식류모형，평고CAI、DEX급기합용조적항종류료효。 ELISA법검측종류균장중종류배사인자-α（ TNF-α）수평。면역형광법대제취적종류내거서세포진행감정。 RT-PCR법검측종류내거서세포중TNF-αmRNA표체수평。결과 CAI억제이식류적생장。동시CAI능강저종류내거서세포중TNF-α적수평（ P＜0.01）。 CAI여소제량DEX연합응용，기억류작용급억제TNF-α수평여단용조상비명현증강（ P＜0.05）。결론 CAI능구억제이식류생장，기작용궤제가능여억제종류중거서세포적TNF-α수평유관。통과여지새미송연합응용，가기도항종류증효작용。
Objective To explore the effect of carboxyamidotriazole ( CAI ) on the Lewis lung carcimoma growth and its underlying mechanisms .Besides,this study proposes whether the combination of CAI and low dose dexamethasone ( DEX) can enhance the anti-tumor effect .Methods A mouse model with transplanted tumor was established to evaluate the anti-cancer effects of the CAI , DEX and their combination .Tumor tissue samples were homogenized followed by ELISA to detect the level of tumor necrosis factor -α( TNF-α) .The macrophages extracted from tumor tissues were stained and indentified by F 4/80 immuno-fluorescent test .TNF-αmRNA expression level was analyzed by RT-PCR.Results CAI could inhibit the growth of mouse transplanted tumor .CAI highly significantly downregulated TNF-αlevel within macrophages from tumor tissues ( P<0.01 ) .The combination of CAI and DEX showed more significant inhibitory effect on the growth of transplanted tumor and downregulated TNF -αlevel than single drug respectively ( P<0.05 ) .Conclusions CAI inhibits tumor growth may cause by suppressing TNF-αproduction in the macrophages from tumor tissues .As those actions of CAI could be enhanced by low dose DEX.