CAJ | 학술논문

目的研究胃癌细胞中CD14的过表达对TNF-α、IL-1β、IL-6、IL-12的影响，探讨CD14在胃癌发生发展中的作用。方法在本实验室前期建立的胃癌SGC-7901 CD14稳定转染细胞系的基础上，利用CD14蛋白受体胞壁酰二肽（ MDP）刺激细胞，RT-PCR检测各细胞因子mRNA的表达，Western blot 检测各细胞因子蛋白的表达，以空质粒转染的胃癌SGC-7901细胞为对照，探讨CD14强制表达对TNF-α、IL-1β、IL-6、IL-12表达的影响。结果转染并稳定表达CD14的细胞中TNF-α、IL-1β、IL-6、IL-12在mRNA及蛋白表达水平上都有不同程度的升高，与空质粒转染的细胞相比，差异均有统计学意义（ P均＜0.05）。结论 CD14对TNF-α、IL-1β、IL-6、IL-12的表达具有一定的促进作用。
목적연구위암세포중CD14적과표체대TNF-α、IL-1β、IL-6、IL-12적영향，탐토CD14재위암발생발전중적작용。방법재본실험실전기건립적위암SGC-7901 CD14은정전염세포계적기출상，이용CD14단백수체포벽선이태（ MDP）자격세포，RT-PCR검측각세포인자mRNA적표체，Western blot 검측각세포인자단백적표체，이공질립전염적위암SGC-7901세포위대조，탐토CD14강제표체대TNF-α、IL-1β、IL-6、IL-12표체적영향。결과전염병은정표체CD14적세포중TNF-α、IL-1β、IL-6、IL-12재mRNA급단백표체수평상도유불동정도적승고，여공질립전염적세포상비，차이균유통계학의의（ P균＜0.05）。결론 CD14대TNF-α、IL-1β、IL-6、IL-12적표체구유일정적촉진작용。
Objective To study the effect of CD14 overexpression in gastric cancer cells on TNF-α,IL-1β, IL-6 and IL-12 and discuss its function in the occurrence and development of gastric cancer .Methods The gastric cancer cells SGC-7901 stably transfected with CD14 were stimulated with muramyl dipeptide ( MDP) of CD14 receptor.mRNA expression and protein expression of each cell factor was detected with RT -PCR and Western blot , respectively .The gastric cancer cells SGC-7901 transfected with empty plasmid was as the control and the effect of enforced CD14 expression on expression of TNF-α,IL-1β,IL-6 and IL-12 was discussed .Results mRNA expression and protein expression of TNF-α,IL-1β,IL-6 and IL-12 were increased in the cells stably transfected with CD 14. Conclusions CD14 plays a role in increasing expression of TNF-α,IL-1β,IL-6 and IL-12 to a certain extent .