CAJ | 학술논문

目的通过观察高频电刺激帕金森(PD)模型大鼠脚桥核（PPN）对苍白球内侧部(GPi)氨基酸类递质及神经元放电的影响，探讨电刺激PPN治疗PD的机制。方法将70只SD雄性大鼠随机分为对照组30只，PD模型组40只。大脑右侧黑质致密部两点注射6-羟基多巴胺建立PD模型，应用细胞外单位记录观察高频电刺激PPN对GPi神经元放电的影响。脑内微透析收集损毁侧GPi脑脊液，结合高效液相色谱检测氨基酸类递质的变化。结果高频电刺激PPN，对照组及PD模型组大鼠GPi神经元的反应均以兴奋性为主，且平均放电频率均较刺激前明显增高（P﹤0.01）；高频电刺激PPN，GPi内谷氨酸水平较刺激前明显减少（P﹤0.01），γ-氨基丁酸水平较刺激前无明显变化（P﹥0.05）。结论高频电刺激PPN可增强GPi神经元的电活动，降低GPi内谷氨酸的水平，其兴奋GPi神经元的效应可能是通过PPN-GPi直接通路或其他间接通路实现的。
목적통과관찰고빈전자격파금삼(PD)모형대서각교핵（PPN）대창백구내측부(GPi)안기산류체질급신경원방전적영향，탐토전자격PPN치료PD적궤제。방법장70지SD웅성대서수궤분위대조조30지，PD모형조40지。대뇌우측흑질치밀부량점주사6-간기다파알건립PD모형，응용세포외단위기록관찰고빈전자격PPN대GPi신경원방전적영향。뇌내미투석수집손훼측GPi뇌척액，결합고효액상색보검측안기산류체질적변화。결과고빈전자격PPN，대조조급PD모형조대서GPi신경원적반응균이흥강성위주，차평균방전빈솔균교자격전명현증고（P﹤0.01）；고빈전자격PPN，GPi내곡안산수평교자격전명현감소（P﹤0.01），γ-안기정산수평교자격전무명현변화（P﹥0.05）。결론고빈전자격PPN가증강GPi신경원적전활동，강저GPi내곡안산적수평，기흥강GPi신경원적효응가능시통과PPN-GPi직접통로혹기타간접통로실현적。
Objective To study the effect of high frequency stimulation (HFS) in pedunculopontine nucleus (PPN) on the neuronal activities of globus pallidus internus (Gpi) in Parkinson’s disease (PD) model rats, and the mechanisms there-of. Methods Seventy male Sprague-Dawley rats were divided into two groups, control group (n=30) and PD model group (n=40). PD rat model was established by the injection of 6-OHDA into substantia nigra pars compacta (SNc) on the right side of the brain with stereotactic technique. Electrophysiological recordings were made in anaesthetized rats to investigate the ef-fects of HFS-PPN on the firing rate of the GPi neurons. Brain microdialysis combined with high-performance liquid chroma-tography was applied to detect glutamate (Glu) andγ-aminobutyric acid (GABA) levels in GPi. Results HFS-PPN caused an excitatory reaction of the majority of neurons recorded in the GPi in PD model group and control group. The mean firing rate of GPi excited neurons was significantly increased (P﹤0.01). The levels of Glu were reduced under HFS-PPN and the levels of GABA were not affected (P>0.05).Conclusion HFS-PPN heightened the electrical activity of GPi neurons and re-duced the level of Glu. These excitatory effects were probably realized by PPN-GPi direct path or other indirect path.