天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2013年
7期
665-667
,共3页
李娟%郝邯生%王悦
李娟%郝邯生%王悅
리연%학함생%왕열
鲍氏不动杆菌%整合子类%多态性,限制性片段长度%聚合酶链反应%插入序列共同区
鮑氏不動桿菌%整閤子類%多態性,限製性片段長度%聚閤酶鏈反應%插入序列共同區
포씨불동간균%정합자류%다태성,한제성편단장도%취합매련반응%삽입서렬공동구
acinetobacter baumannii%integrons%polymorphism,restriction fragment length%polymerase chain re-action%insertion sequence common region
目的了解鲍曼不动杆菌临床株中Ⅰ类整合子、插入序列共同区（ISCR1）的分布情况，研究它们所携带的耐药基因。方法收集来自临床的51株多重耐药鲍曼不动杆菌，PCR检测Ⅰ类整合酶基因、Ⅰ类整合子可变区、ISCR1和ISCR1下游基因环境，DNA序列分析检测Ⅰ类整合子和ISCR1携带的耐药基因，PCR-mapping检测Ⅰ类整合子与ISCR1的关系。结果51株多重耐药鲍曼不动杆菌中Ⅰ类整合酶基因阳性45株，32株检出可变区结构， DNA序列分析显示携带aacA4-catB8-aadA1、aacC1-orfA-orfB-aadA1、blaPSE-1-aadA2-cmlA1-aadA13种形式的耐药基因盒；ISCR1阳性22株，5株检出ISCR1下游基因，DNA序列分析结果为qnrA1-ampR。PCR-mapping显示20株鲍曼不动杆菌中的ISCR1直接连接在Ⅰ类整合子3′保守区下游。结论Ⅰ类整合子、ISCR1在鲍曼不动杆菌耐药中起重要作用，且Ⅰ类整合子和ISCR1可以串联形式存在于鲍曼不动杆菌中。
目的瞭解鮑曼不動桿菌臨床株中Ⅰ類整閤子、插入序列共同區（ISCR1）的分佈情況，研究它們所攜帶的耐藥基因。方法收集來自臨床的51株多重耐藥鮑曼不動桿菌，PCR檢測Ⅰ類整閤酶基因、Ⅰ類整閤子可變區、ISCR1和ISCR1下遊基因環境，DNA序列分析檢測Ⅰ類整閤子和ISCR1攜帶的耐藥基因，PCR-mapping檢測Ⅰ類整閤子與ISCR1的關繫。結果51株多重耐藥鮑曼不動桿菌中Ⅰ類整閤酶基因暘性45株，32株檢齣可變區結構， DNA序列分析顯示攜帶aacA4-catB8-aadA1、aacC1-orfA-orfB-aadA1、blaPSE-1-aadA2-cmlA1-aadA13種形式的耐藥基因盒；ISCR1暘性22株，5株檢齣ISCR1下遊基因，DNA序列分析結果為qnrA1-ampR。PCR-mapping顯示20株鮑曼不動桿菌中的ISCR1直接連接在Ⅰ類整閤子3′保守區下遊。結論Ⅰ類整閤子、ISCR1在鮑曼不動桿菌耐藥中起重要作用，且Ⅰ類整閤子和ISCR1可以串聯形式存在于鮑曼不動桿菌中。
목적료해포만불동간균림상주중Ⅰ류정합자、삽입서렬공동구（ISCR1）적분포정황，연구타문소휴대적내약기인。방법수집래자림상적51주다중내약포만불동간균，PCR검측Ⅰ류정합매기인、Ⅰ류정합자가변구、ISCR1화ISCR1하유기인배경，DNA서렬분석검측Ⅰ류정합자화ISCR1휴대적내약기인，PCR-mapping검측Ⅰ류정합자여ISCR1적관계。결과51주다중내약포만불동간균중Ⅰ류정합매기인양성45주，32주검출가변구결구， DNA서렬분석현시휴대aacA4-catB8-aadA1、aacC1-orfA-orfB-aadA1、blaPSE-1-aadA2-cmlA1-aadA13충형식적내약기인합；ISCR1양성22주，5주검출ISCR1하유기인，DNA서렬분석결과위qnrA1-ampR。PCR-mapping현시20주포만불동간균중적ISCR1직접련접재Ⅰ류정합자3′보수구하유。결론Ⅰ류정합자、ISCR1재포만불동간균내약중기중요작용，차Ⅰ류정합자화ISCR1가이천련형식존재우포만불동간균중。
Objective To investigate the distribution and the antibiotic resistance genes carried by classⅠintegron and insertion sequence common region(ISCR1)among clinical isolates of Acinetobacter baumannii. Methods Fifty-one clinical isolates of multidrug-resistant Acinetobacter baumannii were collected. Polymerase chain reaction method was used to detect the classⅠintegrase gene, variable region of classⅠintegron, ISCR1 and genes related to antibiotics resistance lo-cated downstream of ISCR1 in 51 multidrug-resistant Acinetobacter baumannii. Antibiotics resistance genes carried by classⅠintegron and ISCR1 were performed by DNA sequencing. The relationship between classⅠintegron and ISCR1 was detected by PCR-mapping. Results Among 51 multidrug-resistant Acinetobacter baumannii isolates, 45 strains were found con-taining classⅠintegrase genes, 32 strains were found containing variable regions. Sequencing results showed that the gene cassette arrays were aacA4-catB8-aadA1, aacC1-orfA-orf-B-aadA1 and blaPSE-1-aadA2-cmlA1-aadA1. Twenty-two strains were found containing ISCR1 and 5 strains were found containing resistance genes located downstream of ISCR1. DNA sequencing results showed the resistant gene of qnrA1-ampR. The results of PCR-mapping showed that ISCR1 located directly downstream of 3' conserved segment of classⅠintegron in 20 Acinetobacter baumannii strains. Conclusion ClassⅠintegron and ISCR1 play an important role in mechanisms of drug resistance of Acinetobacter baumannii. ClassⅠintegron and ISCR1 could connect in series in Acinetobacter baumannii.
