CAJ | 학술논문

采用硅胶层析、反相硅胶中压柱层析、高效液相等色谱技术对姜科姜黄属束骨姜黄（ Curcuma xanthorrhiza Roxb．）根茎中的低极性化学成分进行了分离纯化，并通过MS和NMR等波谱技术确定了化合物结构，分离纯化化合物6个，分别鉴定为蓬莪术环氧酮（Ⅰ）、β-谷甾醇（Ⅱ）、愈创木内酯Guai-1（10），3，5，7（11），8-pentaen-2-on-11，8-olide（Ⅲ）、郁金二酮（Ⅳ）、莪术双环烯酮（Ⅴ）、花侧柏烯（Ⅵ）。化合物Ⅲ、Ⅵ为首次从该科植物中分离得到，化合物Ⅳ、Ⅴ为首次从该种中分离得到。对所分离获得化合物进行了乙酰胆碱酯酶抑制活性测定，其中化合物Ⅳ、Ⅵ具有活性，化合物Ⅳ的TLC生物自显影最小抑制量为95μg。
채용규효층석、반상규효중압주층석、고효액상등색보기술대강과강황속속골강황（ Curcuma xanthorrhiza Roxb．）근경중적저겁성화학성분진행료분리순화，병통과MS화NMR등파보기술학정료화합물결구，분리순화화합물6개，분별감정위봉아술배양동（Ⅰ）、β-곡치순（Ⅱ）、유창목내지Guai-1（10），3，5，7（11），8-pentaen-2-on-11，8-olide（Ⅲ）、욱금이동（Ⅳ）、아술쌍배희동（Ⅴ）、화측백희（Ⅵ）。화합물Ⅲ、Ⅵ위수차종해과식물중분리득도，화합물Ⅳ、Ⅴ위수차종해충중분리득도。대소분리획득화합물진행료을선담감지매억제활성측정，기중화합물Ⅳ、Ⅵ구유활성，화합물Ⅳ적TLC생물자현영최소억제량위95μg。
Chemical constituents from Curcuma xanthorrhiza Roxb.( Zingiberaceae) rhizome were isolated and purified by column chromatography ( silica gel, RP-silica gel MPLC, HPLC) and their structures were elucidated on the basis of spectral analyses.Six compounds were identified by MS and NMR spectroscopy as zederone(Ⅰ), β-sitosterol(Ⅱ), Guai-1(10),3,5,7(11),8-pentaen-2-on-11,8-olide(Ⅲ), Curcumadione(Ⅳ), Curcumenone (Ⅴ), Cuparenol (Ⅵ). CompoundⅢand Ⅵwere isolated for the first time from the plants of Zingiberaceae, and compound Ⅳand Ⅴ were isolated from Curcuma xanthorrhiza Roxb.for the first time.Meanwhile, the acetylcholinesterase inhibitory activity of the isolated compounds was evaluated.CompoundsⅣandⅥshowed certain inhibitory activity, the minimum inhibito-ry quantity ofⅣon TLC bioautography test was 95μg.