CAJ | 학술논문

采用农杆菌介导法将含有苏云金芽孢杆菌毒蛋白基因（ CryIAc）与半夏凝集素抗虫基因（ Pta）的高效植物表达载体pCAMBIA3300转入番茄品系Micro Tom的子叶外植体中。经过共培养、除草剂筛选和分化再生，获得了24个具有除草剂抗性的株系。再将转化后的番茄植株经过PCR检测和Southern Blot检测，确定检测后呈阳性反应的株系为8个。通过小菜蛾幼虫初步抗性试验证明，转基因株系表现出较强的抗虫性。实验结果为进一步研究番茄抗虫性和培育抗虫番茄新品种奠定了重要基础。
채용농간균개도법장함유소운금아포간균독단백기인（ CryIAc）여반하응집소항충기인（ Pta）적고효식물표체재체pCAMBIA3300전입번가품계Micro Tom적자협외식체중。경과공배양、제초제사선화분화재생，획득료24개구유제초제항성적주계。재장전화후적번가식주경과PCR검측화Southern Blot검측，학정검측후정양성반응적주계위8개。통과소채아유충초보항성시험증명，전기인주계표현출교강적항충성。실험결과위진일보연구번가항충성화배육항충번가신품충전정료중요기출。
A plant expression vector pCAMBIA3300-Pta-Cry1Ac harboring two insecticidal genes was constructed and employed in Agrobacterium-mediated tomato transformation by using cotyledons as the explants.We harvested 24 phos-phinothricin( PPT0-resistant independent regenerated plantlets after co-culture, selection culture and regeneration.PCR and Southern blot hybridization analyses confirmed that the two target genes were successfully integrated into 8 lines of tomato transformants.Bioassay of transgenic tomatos using insect larvae showed that transgenic plants were conferred with increased insect-resistance compared to untransformed plants.This study laid a potential foundation for the further research on tomato insect-resistance and for the tomato genetic improvement.