浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2013年
8期
1005-1009
,共5页
补肾活血法%骨质疏松症%血瘀%肾虚%痛阈%炎性因子%骨密度
補腎活血法%骨質疏鬆癥%血瘀%腎虛%痛閾%炎性因子%骨密度
보신활혈법%골질소송증%혈어%신허%통역%염성인자%골밀도
Bu Shen Huo Xue method%osteoporosis%blood stasis%kidney deficiency%pain thedhoid%inflammatory factor%BMD
[目的]观察益骨口服液对去势大鼠骨质疏松痛阈、骨密度、血清炎性因子、血管内皮素、血小板活化功能的影响，分析益骨口服液抑制去势大鼠骨质疏松疼痛的作用机制。[方法]将80只SD大鼠随机分为假手术组、益骨口服液组、密盖息组、对照组，每组20只，益骨口服液组、密盖息组、对照组大鼠行卵巢切除术，假手术组行假手术处理,造模成功后,密盖息组予密盖息肌注，假手术组、益骨口服液组、对照组分别予0.9％氯化钠注射液、益骨口服液、0.9％氯化钠注射液等不同药物灌胃,分别测量各组大鼠痛域、骨密度、血清炎性因子COX-2、PGE2、cAMP、血管内皮素、血小板膜糖蛋白CD62p,CD63的含量。[结果]与对照组相比，益骨口服液组去势大鼠骨密度、痛域升高，差异有统计学意义（P<0.05）。与对照组相比，益骨口服液组去势大鼠血清炎性因子COX-2、PGE2、cAMP、血浆内皮素、血小板膜糖蛋白CD62p、CD63的含量降低，差异有统计学意义（P<0.05）。[结论]益骨口服液能提高去势大鼠痛域，抑制大鼠骨质疏松疼痛，其机制与提高骨密度、降低外周血清炎性因子、血管内皮素(ET)、血小板活化功能(CD62p、CD63)等微观分子有关。
[目的]觀察益骨口服液對去勢大鼠骨質疏鬆痛閾、骨密度、血清炎性因子、血管內皮素、血小闆活化功能的影響，分析益骨口服液抑製去勢大鼠骨質疏鬆疼痛的作用機製。[方法]將80隻SD大鼠隨機分為假手術組、益骨口服液組、密蓋息組、對照組，每組20隻，益骨口服液組、密蓋息組、對照組大鼠行卵巢切除術，假手術組行假手術處理,造模成功後,密蓋息組予密蓋息肌註，假手術組、益骨口服液組、對照組分彆予0.9％氯化鈉註射液、益骨口服液、0.9％氯化鈉註射液等不同藥物灌胃,分彆測量各組大鼠痛域、骨密度、血清炎性因子COX-2、PGE2、cAMP、血管內皮素、血小闆膜糖蛋白CD62p,CD63的含量。[結果]與對照組相比，益骨口服液組去勢大鼠骨密度、痛域升高，差異有統計學意義（P<0.05）。與對照組相比，益骨口服液組去勢大鼠血清炎性因子COX-2、PGE2、cAMP、血漿內皮素、血小闆膜糖蛋白CD62p、CD63的含量降低，差異有統計學意義（P<0.05）。[結論]益骨口服液能提高去勢大鼠痛域，抑製大鼠骨質疏鬆疼痛，其機製與提高骨密度、降低外週血清炎性因子、血管內皮素(ET)、血小闆活化功能(CD62p、CD63)等微觀分子有關。
[목적]관찰익골구복액대거세대서골질소송통역、골밀도、혈청염성인자、혈관내피소、혈소판활화공능적영향，분석익골구복액억제거세대서골질소송동통적작용궤제。[방법]장80지SD대서수궤분위가수술조、익골구복액조、밀개식조、대조조，매조20지，익골구복액조、밀개식조、대조조대서행란소절제술，가수술조행가수술처리,조모성공후,밀개식조여밀개식기주，가수술조、익골구복액조、대조조분별여0.9％록화납주사액、익골구복액、0.9％록화납주사액등불동약물관위,분별측량각조대서통역、골밀도、혈청염성인자COX-2、PGE2、cAMP、혈관내피소、혈소판막당단백CD62p,CD63적함량。[결과]여대조조상비，익골구복액조거세대서골밀도、통역승고，차이유통계학의의（P<0.05）。여대조조상비，익골구복액조거세대서혈청염성인자COX-2、PGE2、cAMP、혈장내피소、혈소판막당단백CD62p、CD63적함량강저，차이유통계학의의（P<0.05）。[결론]익골구복액능제고거세대서통역，억제대서골질소송동통，기궤제여제고골밀도、강저외주혈청염성인자、혈관내피소(ET)、혈소판활화공능(CD62p、CD63)등미관분자유관。
[Objective]To observe the influence of Yigu oral liquid on inhibiting the pain in ovariectomized rats by detecing the pain threshold, bone densi-ty, serum inflammatory factor, vascular endothelial function(ET) and platelet activating capacity(CD62p and CD63).[Method]80 SD rats were randomly di-vided into four groups of sham group, Yigu oral liquid group, Miacalcic group,control group. There were 20 in each group.Yigu oral liquid group,Mia-calcic group,control group were ovariectomized,sham group underwent sham operation. After confirming the successful model of osteoporosis, sham group was administrated with saline, Miacalcic saline group was administrated with Miacalcic by intramuscular injection ,Yigu oral liquid group was administrated with Yigu oral liquid ,saline and control group was administrated with saline.The indicators of pain threshold,bone density,vascular endothelial function (ET) and platelet activating capacity(CD62p and CD63), serum inflammatory factors as COX-2, PGE2, cAMP were respectively detected. [Results] The levels of peripheral inflammatory factors as COX-2, PGE2, cAMP , vascular endothelial function(ET) and platelet activating capacity(CD62p and CD63)of Yigu oral liquid group decreased significantly compared with the control group( P<0.05). The level of pain threshold and bone density of Yigu oral liquid group improved significantly compared with the control group(P<0.05).[Conclusion]Yigu oral liquid can inhibit osteoporosis pain by improving the level of pain threshold ,bone density,and decreasing the levels of COX-2, PGE2, cAMP, vascular endothelial function(ET) and platelet activating capacity (CD62p and CD63).
