CAJ | 학술논문

目的对尾静脉注射及皮下接种方法构建的小鼠高表达miR-17-92的L1210白血病模型的生物学特性进行比较研究，选择基本符合高表达miR-17-92的L1210细胞生物学特性，且一致性更好的接种方法。方法常规培养小鼠高表达miR-17-92的L1210系，分别给DBA/2N小鼠以尾静脉注射细胞浓度100×104（A组）、细胞浓度300×104（B组），皮下注射途径接种右上肢根部细胞浓度300×104（C组），观察小鼠成瘤情况，定期计数各组小鼠外周白细胞数量并观察细胞形态改变，取濒死小鼠的肝、脾、肺、肾等脏器称重并制作病理切片。结果各组小鼠成瘤率均为100％， A、B、C 三组平均存活天数分别为：（24.3±3.64） d、（14.2±3.42）d、（26.4±5.56）d。均于接种第8天尾静脉取血，外周血白细胞数目分别为（9.96±2.03）×109/L、（10.81±1.70）×109/L、（12.76±2.06）×109/L，外周血白血病细胞比例分别为（21.33±2.78）％、（32.17±6.5）％、（10.0±2.0）％；接种第15天外周血白细胞数目分别为（8.07±1.31）×109/L、（5.02±1.22）×109/L、（13.28±2.08）×109/L，外周血白血病细胞比例分别为（32.83±3.50）％、（49.67±7.00）％、（12.66±2.00）％。各组濒死小鼠脾有弥漫性白血病细胞浸润，正常组织结构被破坏；肝散在白血病细胞浸润，正常组织结构部分被破坏；肺和肾内有少量白血病细胞浸润，正常组织结构破坏不明显，肺脏有明显出血。结论尾静脉注射及皮下注射高表达miR-17-92的L1210细胞均可使DBA/2小鼠成瘤，尾静脉注射更符合白血病的生物学特点。尾静脉注射高表达miR-17-92的L1210细胞浓度100×104存活时间长，外周血白血病细胞比例较高，适宜观察周期较长的实验。尾静脉注射高表达miR17-92的L1210细胞浓度300×104存活时间短，不适宜观察周期较长的实验；皮下注射可见明显的瘤结节，存活时间最长，适宜观察周期长的实验，但外周血白血病细胞比例偏低。目的對尾靜脈註射及皮下接種方法構建的小鼠高錶達miR-17-92的L1210白血病模型的生物學特性進行比較研究，選擇基本符閤高錶達miR-17-92的L1210細胞生物學特性，且一緻性更好的接種方法。方法常規培養小鼠高錶達miR-17-92的L1210繫，分彆給DBA/2N小鼠以尾靜脈註射細胞濃度100×104（A組）、細胞濃度300×104（B組），皮下註射途徑接種右上肢根部細胞濃度300×104（C組），觀察小鼠成瘤情況，定期計數各組小鼠外週白細胞數量併觀察細胞形態改變，取瀕死小鼠的肝、脾、肺、腎等髒器稱重併製作病理切片。結果各組小鼠成瘤率均為100％， A、B、C 三組平均存活天數分彆為：（24.3±3.64） d、（14.2±3.42）d、（26.4±5.56）d。均于接種第8天尾靜脈取血，外週血白細胞數目分彆為（9.96±2.03）×109/L、（10.81±1.70）×109/L、（12.76±2.06）×109/L，外週血白血病細胞比例分彆為（21.33±2.78）％、（32.17±6.5）％、（10.0±2.0）％；接種第15天外週血白細胞數目分彆為（8.07±1.31）×109/L、（5.02±1.22）×109/L、（13.28±2.08）×109/L，外週血白血病細胞比例分彆為（32.83±3.50）％、（49.67±7.00）％、（12.66±2.00）％。各組瀕死小鼠脾有瀰漫性白血病細胞浸潤，正常組織結構被破壞；肝散在白血病細胞浸潤，正常組織結構部分被破壞；肺和腎內有少量白血病細胞浸潤，正常組織結構破壞不明顯，肺髒有明顯齣血。結論尾靜脈註射及皮下註射高錶達miR-17-92的L1210細胞均可使DBA/2小鼠成瘤，尾靜脈註射更符閤白血病的生物學特點。尾靜脈註射高錶達miR-17-92的L1210細胞濃度100×104存活時間長，外週血白血病細胞比例較高，適宜觀察週期較長的實驗。尾靜脈註射高錶達miR17-92的L1210細胞濃度300×104存活時間短，不適宜觀察週期較長的實驗；皮下註射可見明顯的瘤結節，存活時間最長，適宜觀察週期長的實驗，但外週血白血病細胞比例偏低。
목적대미정맥주사급피하접충방법구건적소서고표체miR-17-92적L1210백혈병모형적생물학특성진행비교연구，선택기본부합고표체miR-17-92적L1210세포생물학특성，차일치성경호적접충방법。방법상규배양소서고표체miR-17-92적L1210계，분별급DBA/2N소서이미정맥주사세포농도100×104（A조）、세포농도300×104（B조），피하주사도경접충우상지근부세포농도300×104（C조），관찰소서성류정황，정기계수각조소서외주백세포수량병관찰세포형태개변，취빈사소서적간、비、폐、신등장기칭중병제작병리절편。결과각조소서성류솔균위100％， A、B、C 삼조평균존활천수분별위：（24.3±3.64） d、（14.2±3.42）d、（26.4±5.56）d。균우접충제8천미정맥취혈，외주혈백세포수목분별위（9.96±2.03）×109/L、（10.81±1.70）×109/L、（12.76±2.06）×109/L，외주혈백혈병세포비례분별위（21.33±2.78）％、（32.17±6.5）％、（10.0±2.0）％；접충제15천외주혈백세포수목분별위（8.07±1.31）×109/L、（5.02±1.22）×109/L、（13.28±2.08）×109/L，외주혈백혈병세포비례분별위（32.83±3.50）％、（49.67±7.00）％、（12.66±2.00）％。각조빈사소서비유미만성백혈병세포침윤，정상조직결구피파배；간산재백혈병세포침윤，정상조직결구부분피파배；폐화신내유소량백혈병세포침윤，정상조직결구파배불명현，폐장유명현출혈。결론미정맥주사급피하주사고표체miR-17-92적L1210세포균가사DBA/2소서성류，미정맥주사경부합백혈병적생물학특점。미정맥주사고표체miR-17-92적L1210세포농도100×104존활시간장，외주혈백혈병세포비례교고，괄의관찰주기교장적실험。미정맥주사고표체miR17-92적L1210세포농도300×104존활시간단，불괄의관찰주기교장적실험；피하주사가견명현적류결절，존활시간최장，괄의관찰주기장적실험，단외주혈백혈병세포비례편저。
Objective To make a comparative study of biological characteristics of a transferable mouse L1210 leukemia model with higher expression of miR-17-92 inoculated by tail intravenous injection and hypodermic inoculation in order to select the best inoculation method that fits in with the bionomics of L 1210 cells and has a better consistency .Methods B-leukemia cell line L1210 was conventionally infused into each DBA/2N mouse through the vena caudalia at the cellular concentration of 100 ×104(A group),at the cellular concentration of 300 × 104(B group),and subcutis at the cellular concentration of 300 ×104 in the initial part of right leg(C group).The process of leukemia in these mice was investigated .The amount of peripheral leucocytes in mouse blood was counted and the morphological changes were investigated .The liver,spleen,kidney and lung of the dying mice were kept , weighed and made into pathological sections .Results The rate of tumorigenesis was 100%in each group .The average survival time of the three groups was (24.3 ±3.64)d,(14.2 ±3.42)d,(26.4 ±5.56)d.The amount of peripheral leucocytes increased remarkably after 7 days infusion was(9.96 ±2.03) ×109/L,(10.81 ±1.70) ×109/L,(12.76 ±2.06) ×109/L,and the ratio of Leukemia cell was (21.33 ±2.78)%,(32.17 ±6.5)%,(10 ±2)%. After 14 days infusion it was(8.07 ±1.31) ×109/L,(5.02 ±1.22) ×109/L,(13.28 ±2.08) ×109/L,and the ratio was(32.83 ±3.50 )%,(49.67 ±7.00 )%,(12.66 ±2.00 )%.Leukemia cells could be found in peripheral blood smears of the three groups .In pathological Sections of the liver and spleen of dying mice ,diffused leukemia cell infiltration could be observed , accompanied by destruction to the normal tissue and structure .A small quantity of leukemia cells was found in lungs and kidneys .The destruction to their normal tissue and structure was not obvious and hemorrhage was easily seen in lungs .Conclusions Inoculation of L1210 cells with higher expression of miR-17-92 into DBA/2N mice through the vena caudalia or subeufis is feasible for developing a mouse leukemia model ,and the method of vena caudalia fits in with the bionomics of L 1210 cells and has a better consistency .By tail intravenous injection,the survival time in tumor is short and not apt for long time experiment observation .By hypodermic injection,the survival time in tumor is long and tumor nodules are visible , and apt for long time experiment observation ,but the ratio of lymphoma cell was not high .