浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2013年
8期
951-955
,共5页
阿霉素%胆固醇基普鲁兰多糖%自组装纳米粒%体外释放%抗肿瘤
阿黴素%膽固醇基普魯蘭多糖%自組裝納米粒%體外釋放%抗腫瘤
아매소%담고순기보로란다당%자조장납미립%체외석방%항종류
adriamycin%cholesterol-modified pul ulan%self-assembled nanoparticles%in vitro release%antitumor
[目的]制备胆固醇基普鲁兰多糖阿霉素自组装纳米粒（Self-assembled ADM-loaded cholesterol modified pul ulan nanoparticles, ADM-CHSP-SAN）并考察其体外抗肿瘤活性。[方法]以胆固醇基普鲁兰多糖（cholesterol-modified pul ulan，CHSP）为载体，采用透析法制备ADM-CHSP-SAN，并测定其形态、粒径、Zeta电位、包封率和载药量，采用MTT法研究其抑制U251肿瘤细胞的活性作用。[结果]ADM-CHSP-SAN外观呈圆形或类圆形，平均粒径为（112.8±1.02）nm，Zeta电位为（-27.2±0.246）mV，包封率和载药量分别为（67.14±1.21）%和（7.65±0.58）%；体外释药行为符合Higuchi方程；给药剂量大于25μg·mL-1时，ADM-CHSP-SAN抑制U251肿瘤细胞的活性作用明显优于阿霉素溶液剂（P<0.01）。[结论]将阿霉素制备成ADM-CHSP-SAN可有效提高药物的抗肿瘤活性。
[目的]製備膽固醇基普魯蘭多糖阿黴素自組裝納米粒（Self-assembled ADM-loaded cholesterol modified pul ulan nanoparticles, ADM-CHSP-SAN）併攷察其體外抗腫瘤活性。[方法]以膽固醇基普魯蘭多糖（cholesterol-modified pul ulan，CHSP）為載體，採用透析法製備ADM-CHSP-SAN，併測定其形態、粒徑、Zeta電位、包封率和載藥量，採用MTT法研究其抑製U251腫瘤細胞的活性作用。[結果]ADM-CHSP-SAN外觀呈圓形或類圓形，平均粒徑為（112.8±1.02）nm，Zeta電位為（-27.2±0.246）mV，包封率和載藥量分彆為（67.14±1.21）%和（7.65±0.58）%；體外釋藥行為符閤Higuchi方程；給藥劑量大于25μg·mL-1時，ADM-CHSP-SAN抑製U251腫瘤細胞的活性作用明顯優于阿黴素溶液劑（P<0.01）。[結論]將阿黴素製備成ADM-CHSP-SAN可有效提高藥物的抗腫瘤活性。
[목적]제비담고순기보로란다당아매소자조장납미립（Self-assembled ADM-loaded cholesterol modified pul ulan nanoparticles, ADM-CHSP-SAN）병고찰기체외항종류활성。[방법]이담고순기보로란다당（cholesterol-modified pul ulan，CHSP）위재체，채용투석법제비ADM-CHSP-SAN，병측정기형태、립경、Zeta전위、포봉솔화재약량，채용MTT법연구기억제U251종류세포적활성작용。[결과]ADM-CHSP-SAN외관정원형혹류원형，평균립경위（112.8±1.02）nm，Zeta전위위（-27.2±0.246）mV，포봉솔화재약량분별위（67.14±1.21）%화（7.65±0.58）%；체외석약행위부합Higuchi방정；급약제량대우25μg·mL-1시，ADM-CHSP-SAN억제U251종류세포적활성작용명현우우아매소용액제（P<0.01）。[결론]장아매소제비성ADM-CHSP-SAN가유효제고약물적항종류활성。
[Objective] To prepare adriamycin self-assembled nanoparticles, and study the in vivo anti-tumor activity. [Methods]The self-assembled adri-amycin loaded cholesterol-modified pul ulan nanoparticles were prepared by dialysis and were characterized by morphology for particle size,Zeta potential, entrapment efficiency,drug loading content.They were incubated with U251 cel s to assess the inhibition ability of the self-assembled adriamycin-loaded cholesterol-modified pul ulan nanoparticles. [Results]The morphology of self-assembled adriamycin loaded cholesterol-modified pul ulan nanoparticles was spherical. The mean particle size, Zeta potential, entrapment efficiency and drug loading were (112.8 ±1.02)nm,(-27.2±0.246)mV,(67.14±1.21)% and (7.65±0.58)%, respectively.The profiles of release were expressed wel by Higuchi equation. When the dosages were 25μg·mL-1 plus, the inhibiton ability against U251 was stronger than adriamycin solution( P<0.01).[Conclusion]The self-assembled adriamycin loaded cholesterol-modified pul ulan nanoparticles exhibited more cycitoxic activity against U251 than adriamycin solution.
