CAJ | 학술논문

目的探讨4-HPR 联合顺铂对人卵巢癌细胞株 SKOV3增殖和 Akt/Bax 表达的影响。方法 MTT法检测单独使用4-HPR、顺铂及联合使用对SKOV3细胞增殖的影响；用流式细胞仪检测4-HPR、顺铂对SKOV3细胞周期和Akt/Bax表达的影响。结果顺铂联合不同浓度的4-HPR可以显著抑制SKOV3细胞的增殖，同单独使用顺铂相比抑制作用较强，差异显著（ P＜0.05）。顺铂联合4-HPR同使用顺铂相比可以使G0～G1期细胞比例进一步下降，S期和G2～M期细胞比例进一步上升，差异显著（ P＜0.01）。顺铂联合4-HPR的凋亡率同单独用顺铂组相比差异有统计学意义（ P＜0.01）。顺铂联合不同浓度的4-HPR时可以使SKOV3细胞Akt的表达下调和促进Bax的表达的上调，同单独使用顺铂相比差异有统计学意义（ P＜0.01）。结论顺铂联合4-HPR可以更好地抑制细胞株SKOV3增殖，使得细胞停滞在S期和G2～M期，这可能与可以促进Akt的表达下调和Bax的表达上调有关。
목적탐토4-HPR 연합순박대인란소암세포주 SKOV3증식화 Akt/Bax 표체적영향。방법 MTT법검측단독사용4-HPR、순박급연합사용대SKOV3세포증식적영향；용류식세포의검측4-HPR、순박대SKOV3세포주기화Akt/Bax표체적영향。결과순박연합불동농도적4-HPR가이현저억제SKOV3세포적증식，동단독사용순박상비억제작용교강，차이현저（ P＜0.05）。순박연합4-HPR동사용순박상비가이사G0～G1기세포비례진일보하강，S기화G2～M기세포비례진일보상승，차이현저（ P＜0.01）。순박연합4-HPR적조망솔동단독용순박조상비차이유통계학의의（ P＜0.01）。순박연합불동농도적4-HPR시가이사SKOV3세포Akt적표체하조화촉진Bax적표체적상조，동단독사용순박상비차이유통계학의의（ P＜0.01）。결론순박연합4-HPR가이경호지억제세포주SKOV3증식，사득세포정체재S기화G2～M기，저가능여가이촉진Akt적표체하조화Bax적표체상조유관。
Objective To study the effect of 4-HPR and DDP on proliferation and expression of Akt/Bax in human ovarian cell line SKOV3.Methods The effect of 4-HPR and DDP on SKOV3 cell proliferation was measured by MTT assay.The cell cycle was analyzed by flow cytometry (FCM).The Akt/Bax expressions of SKOV3 cells were detected by FCM.Results DDP combined with 4-HPR (1,5 and 10 μmol/L) significantly inhibited the proliferation of ovarian cancer SKOV3 cells,which had a statistical difference compared with the only-used DDP group (P<0.05).4-HPR combined with DDP significantly arrested SKOV3 cell at G2-M and S stage compared with only-used DDP group ( P<0.01 ) .Apoptosis rate of 4-HPR combined with DDP group was significantly higher than only-used DDP group ( P<0.01 ) .4-HPR combined with DDP affected the expressions of Akt/Bax compared with only-used DDP group ( P <0.01 ) .Conclusion To use 4-HPR combined with DDP can significantly inhibit the proliferation and block cell cycle of ovarian cancer SKOV 3 cells,which is associated with Akt/Bax expressions .