CAJ | 학술논문

目的探讨尼古丁对大鼠骨折愈合及血管内皮生长因子表达的影响。方法60只雄性SD大鼠，随机分为模型组、轻度尼古丁组、重度尼古丁组。制造骨折前后尼古丁生物效应，无菌状态下手术，在大鼠桡骨中下1/3交界处造横断骨折愈合模型；于术后3、7、14、21 d每组随机处死5只大鼠，取左侧桡骨标本作为观察对象，使用HE染色法检测骨痂厚度及成熟度和免疫组化法检测骨痂中VEGF表达水平三个指标。结果造模后第3天，各给药组与模型组比较，标本骨痂厚度及VEGF阳性细胞数差异无统计学意义（ P＞0.05），显微镜下骨痂成熟度无差异；造模后第7天、第14天和第21天，各给药组与模型组比较，标本骨痂厚度及VEGF阳性细胞数差异有统计学意义（P＜0.05，P＜0.01），给药组的VEGF表达高于模型组，但是给药组骨痂厚度低及骨痂成熟度较模型组低；轻度尼古丁组、重度尼古丁组VEGF阳性细胞数组间差异有统计学意义（ P＜0.05），轻度尼古丁组VEGF表达均高于重度尼古丁组。结论尼古丁影响骨痂增殖分化使骨痂的形成减少，抑制骨的成熟改造，而延缓骨折的愈合过程；另一方面尼古丁可促进血管内皮VEGF表达，而可能促进内皮细胞增生从而促进血管新生，而且不同水平的尼古丁对VEGF表达影响不同。
목적탐토니고정대대서골절유합급혈관내피생장인자표체적영향。방법60지웅성SD대서，수궤분위모형조、경도니고정조、중도니고정조。제조골절전후니고정생물효응，무균상태하수술，재대서뇨골중하1/3교계처조횡단골절유합모형；우술후3、7、14、21 d매조수궤처사5지대서，취좌측뇨골표본작위관찰대상，사용HE염색법검측골가후도급성숙도화면역조화법검측골가중VEGF표체수평삼개지표。결과조모후제3천，각급약조여모형조비교，표본골가후도급VEGF양성세포수차이무통계학의의（ P＞0.05），현미경하골가성숙도무차이；조모후제7천、제14천화제21천，각급약조여모형조비교，표본골가후도급VEGF양성세포수차이유통계학의의（P＜0.05，P＜0.01），급약조적VEGF표체고우모형조，단시급약조골가후도저급골가성숙도교모형조저；경도니고정조、중도니고정조VEGF양성세포수조간차이유통계학의의（ P＜0.05），경도니고정조VEGF표체균고우중도니고정조。결론니고정영향골가증식분화사골가적형성감소，억제골적성숙개조，이연완골절적유합과정；령일방면니고정가촉진혈관내피VEGF표체，이가능촉진내피세포증생종이촉진혈관신생，이차불동수평적니고정대VEGF표체영향불동。
Objective To explore the effects of nicotine on rat fracture healing and vascular endothelial growth factor (VEGF) expression.Methods 60 male Sprague-Dawley rats were randomly divided into model group , mild nicotine group and severe nicotine group .Manufacture of biological effects of nicotine;surgery under sterile conditions,causing a transverse fracture healing model in the junction of the lower 1/3 of the rat radial;3,7,14,21 days after surgery ,5 rats each group were sacrificed randomly ,take the left radial specimens as the observed object , using HE staining detection the callus thickness and maturity , immunohistochemical method detection callus VEGF expression level .Results 3 days after modeling , the difference in specimens VEGF positive cells and callus thickness between each treatment group and the model group was not statistically significant ( P>0.05 ) ,no difference in the maturity of the callus under the microscope;7,14 days and 21 days after modeling,the difference in specimens VEGF positive cells and callus thickness between each treatment group and the model group was statistically significant(P<0.05,P<0.01),in the expression of VEGF,treatment group was higher than model group ,but the treatment group callus thickness and maturity was low compared with the model group;the difference in specimens VEGF positive cells between the the mild nicotine group and severe nicotine group was also statistically significant ( P<0.05 ) .Mildnicotine group were higher than the expression of VEGF in the severe nicotine group . Conclusions Nicotine affects the proliferation and differentiation of callus reduced callus formation , inhibition of bone maturity transformation , and delays the healing process of fracture;On the other hand , nicotine may promote vascular endothelial VEGF expression , which may promote the proliferation of endothelial cells to promote angiogenesis ,the different levels of nicotine on VEGF expression impact are also different .