CAJ | 학술논문

目的通过分析调控TNF-α水平的G-308A和G-238A单核苷酸多态性（ SNP）在正常和多囊卵巢综合征患者的分布频率，探讨G-308 A、G-238 A位点SNP与多囊卵巢综合征的关系。方法144例多囊卵巢综合征及72例健康对照妇女，分别测定身高（Ht）、体重（Wt）、腰围（WC）、臀围（HC）、血清甘油三酯（ TG）、总胆固醇（ TC）、高密度脂蛋白胆固醇（ HDL-C）和低密度脂蛋白胆固醇（ LDL-C）、空腹血糖（ FPG）、空腹胰岛素（FINS）、黄体生成素（LH）、卵泡刺激素（FSH）、睾酮（T）、游离睾酮（FT）、TNF-α水平，计算体重指数（BMI）、腰臀比（WHR）、胰岛素抵抗指数（HOMA-IR）、黄体生成素比卵泡刺激素（LH/FSH）。应用PCR-RFLP方法检测TNF-α基因G-308 A及G-238 A位点多态性。结果多囊卵巢综合征患者TNF-α水平高于对照组（ P＜0.05）。多囊卵巢综合征组和对照组TNF-α基因G-308 A位点等位基因A的突变率分别为6.8％与5.9％，两组间基因频率和基因型频率差异无统计学意义；多囊卵巢综合征患者TNF-α基因G-308 A位点各基因型间HOMA-IR差异有统计学意义，但其他临床指标差异均无统计学意义。多囊卵巢综合征组和对照组TNF-α基因G-238 A位点等位基因A的突变率分别为2.4％与2.8％，两组间基因频率和基因型频率差异无统计学意义；多囊卵巢综合征患者TNF-α基因G-238 A位点各基因型间TNF-α差异有统计学意义，但其他临床指标差异均无统计学意义。结论 TNF-α基因G-308 A、G-238 A位点的SNP与多囊卵巢综合征发病可能无明显相关性。
목적통과분석조공TNF-α수평적G-308A화G-238A단핵감산다태성（ SNP）재정상화다낭란소종합정환자적분포빈솔，탐토G-308 A、G-238 A위점SNP여다낭란소종합정적관계。방법144례다낭란소종합정급72례건강대조부녀，분별측정신고（Ht）、체중（Wt）、요위（WC）、둔위（HC）、혈청감유삼지（ TG）、총담고순（ TC）、고밀도지단백담고순（ HDL-C）화저밀도지단백담고순（ LDL-C）、공복혈당（ FPG）、공복이도소（FINS）、황체생성소（LH）、란포자격소（FSH）、고동（T）、유리고동（FT）、TNF-α수평，계산체중지수（BMI）、요둔비（WHR）、이도소저항지수（HOMA-IR）、황체생성소비란포자격소（LH/FSH）。응용PCR-RFLP방법검측TNF-α기인G-308 A급G-238 A위점다태성。결과다낭란소종합정환자TNF-α수평고우대조조（ P＜0.05）。다낭란소종합정조화대조조TNF-α기인G-308 A위점등위기인A적돌변솔분별위6.8％여5.9％，량조간기인빈솔화기인형빈솔차이무통계학의의；다낭란소종합정환자TNF-α기인G-308 A위점각기인형간HOMA-IR차이유통계학의의，단기타림상지표차이균무통계학의의。다낭란소종합정조화대조조TNF-α기인G-238 A위점등위기인A적돌변솔분별위2.4％여2.8％，량조간기인빈솔화기인형빈솔차이무통계학의의；다낭란소종합정환자TNF-α기인G-238 A위점각기인형간TNF-α차이유통계학의의，단기타림상지표차이균무통계학의의。결론 TNF-α기인G-308 A、G-238 A위점적SNP여다낭란소종합정발병가능무명현상관성。
Objective To investigate the relationships between tumor necrosis factor-alpha ( TNF-α) gene G-308 A and G-238 A polymorphisms with the PCOS ,analyze the distribution frequency of TNF-αgene SNP-308 and G-238A in healthy control women and the PCOS .Methods One hundred and fourty-four women ( aged 18 to 40 years) with PCOS and seventy-two healthy control women were compared in this study .Height (Ht),weight(Wt), waist circumference ( WC ) , hip circumference ( HC ) , triglycerides ( TG ) , total cholesterol ( TC ) , high density lipoprotein-cholesterol ( HDL-C ) , low density lipoprotein-cholesterol ( LDL-C ) , fasting plasma glucose ( FPG ) , plasma fasting insulin ( FINS ) , luteinizing hormone ( LH ) , follicle stimulating hormone ( FSH ) , total testosterone (T),free testosterone and TNF-αwere tested.The BMI,waist-to-hip ratio (WHR),homeostasis model assessment for insulin resistance ( HOMA-IR) and LH/FSH were calculated .TNF-αgene-308 and-238 polymorphism were assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results The plasma TNF-αlevels increased in women with PCOS compared to control group ,the A allelic frequency of TNF-αgene SNP-308 in women with PCOS and control group were 6.8% and 5.9% respectively .There were no significant differences in allele or genotype frequency of SNP-308 between women with PCOS and control group ( P>0.05 ) .The HOMA-IR in PCOS women with wild homozygous GG and carriers of A allele of TNF-αpolymorphism gene showed statistical differences while BMI , WHR, FPG, TG, TC, HDL-C, LDL-C, FINS, LH, FSH, LH/FSH, T, FT and TNF-αdid not show statistical differences .The A allelic frequency of TNF-αgene SNP-238 in women with PCOS and control group were 2.4%and 2.8%respectively .There were no significant differences in allele or genotype frequency of SNP -238 between women with PCOS and control group ( P>0.05 ) .The TNF-αin PCOS women with wild homozygous GG and carriers of A allele of TNF-αpolymorphism gene showed statistical differences while Other clinical parameters did not show statistical differences .Conclusions There is no association between TNF-αgene SNP-308 ,TNF-αgene SNP-238 and PCOS .