CAJ | 학술논문

目的明确G蛋白偶联受体34（G-protein coupled receptor 34，GPR34）转录变异体在结肠正常以及癌细胞和组织中的表达模式，探讨其在结肠癌中的潜在作用。方法（1）通过检索NCBI有关GPR34的基因信息和设计特异性引物，利用PCR技术确定结肠中表达的GPR34转录变异体；（2）通过染料法Real-time PCR明确GPR34各转录变异体在结肠正常和肿瘤细胞以及临床组织标本中的定量表达谱；（3）分析GPR34转录变异体表达与结肠癌临床病理特征的相关性。结果（1）结合GPR34基因相关信息分析，通过PCR技术确定了在结肠正常细胞中仅表达序列号为AF039686.1和AK122945.1的转录变异体；（2）与正常的结肠细胞相比，本研究所使用的所有结肠癌细胞中总的GPR34转录变异体和AK122945.1均表达上调；对30例结肠癌临床组织标本的分析表明，结肠癌组织中GPR34总的转录变异体表达上调的有18例，转录变体AK122945.1表达上调的有19例；（3）Fisher精确检验和Spearman相关性统计分析结果显示，GPR34总转录变异体和AK122945.1的表达上调与结肠癌的临床分期呈显著正相关性（ P＜0.05），而与性别、年龄、病理分级和浸润程度无相关性（ P＞0.05）。此外，二者表达上调的患者虽然淋巴结转移的发生趋于增多，但是统计学无差异（ P＞0.05）。结论 GPR34总转录变异体和AK122945.1表达上调涉及结肠癌的发生和发展，其中后者是GPR34总转录变异体表达上调的主要原因，推测其可能具有潜在的促癌作用。
목적명학G단백우련수체34（G-protein coupled receptor 34，GPR34）전록변이체재결장정상이급암세포화조직중적표체모식，탐토기재결장암중적잠재작용。방법（1）통과검색NCBI유관GPR34적기인신식화설계특이성인물，이용PCR기술학정결장중표체적GPR34전록변이체；（2）통과염료법Real-time PCR명학GPR34각전록변이체재결장정상화종류세포이급림상조직표본중적정량표체보；（3）분석GPR34전록변이체표체여결장암림상병리특정적상관성。결과（1）결합GPR34기인상관신식분석，통과PCR기술학정료재결장정상세포중부표체서렬호위AF039686.1화AK122945.1적전록변이체；（2）여정상적결장세포상비，본연구소사용적소유결장암세포중총적GPR34전록변이체화AK122945.1균표체상조；대30례결장암림상조직표본적분석표명，결장암조직중GPR34총적전록변이체표체상조적유18례，전록변체AK122945.1표체상조적유19례；（3）Fisher정학검험화Spearman상관성통계분석결과현시，GPR34총전록변이체화AK122945.1적표체상조여결장암적림상분기정현저정상관성（ P＜0.05），이여성별、년령、병리분급화침윤정도무상관성（ P＞0.05）。차외，이자표체상조적환자수연림파결전이적발생추우증다，단시통계학무차이（ P＞0.05）。결론 GPR34총전록변이체화AK122945.1표체상조섭급결장암적발생화발전，기중후자시GPR34총전록변이체표체상조적주요원인，추측기가능구유잠재적촉암작용。
Objective To determine the expression profile of G-protein coupled receptor 34 ( GPR34 ) transcript variants in colon cancer cell lines and tissues and explore the potential roles of these transcript variants in colon cancer .Methods ( 1 ) By analysis GPR34 candidate transcript variants in NCBI database and designing specific PCR primers ,PCR was used to determine the species of GPR 34 transcript variants were expressed in colon normal and cancer cells or/and tissues;(2) Real-time PCR was used to quantitatively describe the expression profile of GPR34 transcript variants in colon normal and cancer cells and tissues;(3) We performed a statistical analysis of the potential correlation between GPR 34 transcript variants expression and the patients′clinicopathological characteristics .Results ( 1 ) NCBI database analysis and qualitative PCR assay showed us that only two GPR 34 transcript variants,which accession number was AF039686.1 and AK122945.1,respectively,were expressed in the colon normal and cancer cells .(2) Both overall GPR34 transcript variants and AK122945.1 were significantly up-regulated in cancer cells and tissues compared with normal pairs , and among them , AK122945.1 up-regulation is mainly correspond to increased expression of overall GPR 34 transcript variants.(3) Both overall GPR34 transcript variants and AK122945.1 expression in 30 patients was significantly correlated with clinical stage (P<0.05),but not with sex,age,grade,infiltration,and lymph node status (P>0.05).Conclusions These results indicate that up-regulation of overall GPR34 transcript variants and AK122945.1 might act as an potential pro-oncogene to involve in the development and progression of colon cancer and its 5′UTR might be a potential target for therapeutic intervention .