CAJ | 학술논문

目的本研究旨在探讨miR-1和PIK3CA在非小细胞肺癌（NSCLC）组织中的表达及其临床意义。方法利用RT-qPCR法检测55例NSCLC组织及其癌旁正常组织中miR-1和PIK3CA 的表达，并用Western-blot法检测NSCLC组织及其正常组织中PI3K蛋白的表达变化。结果 miR-1在NSCLC原发灶中的表达明显低于在癌旁和正常组织中的表达（P＝0.0122），PIK3CA在NSCLC原发灶和癌旁组织中的表达都明显高于在正常组织中的表达（P＝0.0446，P＜0.0001），且PIK3CA在原发灶中的表达明显高于在癌旁组织中的表达（P＝0.0029）；miR-1和PIK3CA在术后1年内发生/不发生转移癌组织中的表达差异具有统计学意义（P＝0.0369，P＝0.0061）；PIK3CA的表达与NSCLC组织学类型相关（P＝0.0200），但miR-1和PIK3CA的表达与患者的年龄、性别、肿瘤的大小及分化程度无明显相关关系（P＞0.05），miR-1的表达与PIK3CA的表达成负相关（r＝-0.7096，P＜0.05）。结论 miR-1的低表达和PIK3CA的高表达可能和NSCLC的发生及侵袭转移有关，且miR-1和PIK3CA在NSCLC转移过程中相互抑制，可能成为NSCLC治疗的新的潜在靶点。
목적본연구지재탐토miR-1화PIK3CA재비소세포폐암（NSCLC）조직중적표체급기림상의의。방법이용RT-qPCR법검측55례NSCLC조직급기암방정상조직중miR-1화PIK3CA 적표체，병용Western-blot법검측NSCLC조직급기정상조직중PI3K단백적표체변화。결과 miR-1재NSCLC원발조중적표체명현저우재암방화정상조직중적표체（P＝0.0122），PIK3CA재NSCLC원발조화암방조직중적표체도명현고우재정상조직중적표체（P＝0.0446，P＜0.0001），차PIK3CA재원발조중적표체명현고우재암방조직중적표체（P＝0.0029）；miR-1화PIK3CA재술후1년내발생/불발생전이암조직중적표체차이구유통계학의의（P＝0.0369，P＝0.0061）；PIK3CA적표체여NSCLC조직학류형상관（P＝0.0200），단miR-1화PIK3CA적표체여환자적년령、성별、종류적대소급분화정도무명현상관관계（P＞0.05），miR-1적표체여PIK3CA적표체성부상관（r＝-0.7096，P＜0.05）。결론 miR-1적저표체화PIK3CA적고표체가능화NSCLC적발생급침습전이유관，차miR-1화PIK3CA재NSCLC전이과정중상호억제，가능성위NSCLC치료적신적잠재파점。
Objective To explore the expression of miR-1 and PIK3CA in NSCLC, and the relationship between miR-1 and PIK3CA expression and clinical pathology of NSCLC .Methods The expression of miR-1 and PIK3CA was detected in 55 NSCLC tissues,adjacent cancer tissues and normal tissues by RT-PCR.The expression level of PI3K protein was detected by Western-blot.The correlation between miR-1 and PIK3CA expression was analyzed .Results miR-1 expression in cancer tissues was significantly lower than that in adjacent cancer tissues and normal tissues(P=0.0122).The expression of PIK3CA in cancer tissues and adjacent cancer tissue was significantly higher than that in normal tissues(P=0.0446,P<0.0001),and the positive rate of PIK3CA expression in cancer tissues was significantly higher than that in adjacent cancer tissues (P=0.0029).miR-1 and PIK3CA expression in patients with postoperative metastasis within one year was significantly different from patients without postoperative metastasis(P=0.0369,P=0.0061 respectively).PIK3CA expression had remarkable correlation with squamous cell carcinoma(P=0.0200),but miR-1 and PIK3CA expression had no remarkable correlation with age ,sex,tumour size and differentiation(P>0.05).miR-1 and PIK3CA expression in NSCLC was negatively correlated (r=-0.7096, P<0.05).Conclusion The deficiency of miR-1 and PIK3CA expression is positively correlated with carcinogenesis and metastasis of NSCLC .miR-1 and PIK3CA may become a new potential targets for NSCLC treatment .