CAJ | 학술논문

目的对护骨胶囊水提液的多种成分进行定性分析与鉴别,为护骨胶囊水提液质量控制、药理药效研究提供依据。方法采用高效液相色谱-二极管阵列检测器(HPLC-DAD)检测,Kromasil C18色谱柱,流动相为乙腈-1%冰醋酸,流速0.8 mL/min,梯度洗脱,通过与相关药材色谱图进行比对,确认护骨胶囊水提液指纹图谱中各成分的归属。结果建立的高效液相色谱图中可检测出13个特征峰,其中4个色谱峰可明确化学成分的归属。结论建立的HPLC-DAD 图谱表达了护骨胶囊水提液可测组分的整体特征,可用于护骨胶囊水提液的 HPLC 定性考察,提高该复方制剂质量控制的专属性。
목적대호골효낭수제액적다충성분진행정성분석여감별,위호골효낭수제액질량공제、약리약효연구제공의거。방법채용고효액상색보-이겁관진렬검측기(HPLC-DAD)검측,Kromasil C18색보주,류동상위을정-1%빙작산,류속0.8 mL/min,제도세탈,통과여상관약재색보도진행비대,학인호골효낭수제액지문도보중각성분적귀속。결과건립적고효액상색보도중가검측출13개특정봉,기중4개색보봉가명학화학성분적귀속。결론건립적HPLC-DAD 도보표체료호골효낭수제액가측조분적정체특정,가용우호골효낭수제액적 HPLC 정성고찰,제고해복방제제질량공제적전속성。
Objective To analyze and identify the chemical composition in water extracts from Hugu capsule and provide evidence for its pharmacological study and quality control. Methods A HPLC-DAD method was used. The separation was performed on Kromasil C18 column with acetonitrile and 1%glacial acetic acid as mobile phase by gradient elution at a flow rate of 0.8 mL/min. The chemical composition in water extracts from Hugu capsule were identified by comparison with the related chromatographic fingerprint. Results Thirteen characteristic peaks were found in the HPLC chromatographic fingerprint, and four peaks were identified. Conclusion The HPLC-DAD fingerprint expressed the general character of the chemical composition in water extracts from Hugu capsule. It can be used for qualitative analysis of water extracts from Hugu capsule, and improve the quality of Hugu capsule.