CAJ | 학술논문

目的从佛手中筛选对主动脉血管有舒张作用的有效成分。方法采用大鼠血管平滑肌细胞膜色谱模型(VSM/CMC)筛选，液相色谱-质谱(LC/MS)离线分析，并结合离体药理实验确定佛手舒张血管的有效成分。结果佛手柑内酯是佛手中作用于血管的活性成分，药理实验结果表明其具有舒张血管作用，化合物在VSM/CMC的保留特性和其药理作用有显著相关性。结论 VSM/CMC 模型可以反映化合物与细胞膜及膜蛋白(包括受体)的相互作用；VSM/CMC 和LC/MS技术联用，为从天然产物中快速寻找新的活性成分提供了途径。
목적종불수중사선대주동맥혈관유서장작용적유효성분。방법채용대서혈관평활기세포막색보모형(VSM/CMC)사선，액상색보-질보(LC/MS)리선분석，병결합리체약리실험학정불수서장혈관적유효성분。결과불수감내지시불수중작용우혈관적활성성분，약리실험결과표명기구유서장혈관작용，화합물재VSM/CMC적보류특성화기약리작용유현저상관성。결론 VSM/CMC 모형가이반영화합물여세포막급막단백(포괄수체)적상호작용；VSM/CMC 화LC/MS기술련용，위종천연산물중쾌속심조신적활성성분제공료도경。
Objective To screen the active component from Fructus Citri Sarcodactylis which have aortic vascular relaxant effect. Methods The active components from Fructus Citri Sarcodactylis were screened by combining vascular smooth muscle/cell membrane chromatography (VSM/CMC) and liquid chromatography/mass spectrometry (LC/MS) with pharmacological assay in vitro. Results Bergapten was the active component of Fructus Citri Sarcodactylis that functions in vascular. The pharmacological experiment showed that bergapten had vasodilating effect. The capacity factor of this compound on the VSM/CMC model was found to be significantly correlated with its pharmacological effect. Conclusion Association between compound and membrane protein (receptor) can be reflected by using the VSM/CMC model. VSM/CMC-offline-LC/MS technology can be used to quickly screening new active components from natural medicine.