CAJ | 학술논문

目的建立痔瘘洗液的质量标准。方法采用薄层色谱法鉴别痔瘘洗液中的大黄、黄柏,采用高效液相色谱法测定该洗液中绿原酸的含量。色谱柱：Agilent Ecilipse XDB-C18(4.6 mm×250 mm,5μm)；流动相：乙腈-0.4%磷酸溶液(10∶90)；流速：0.80 mL/min；柱温：室温；检测波长：327 nm。结果薄层鉴别方法专属性强,重复性好,空白对照无干扰。绿原酸在2.442～122.1μg范围内呈良好的线性关系(r＝1.0000),平均加样回收率为100.30%, RSD＝1.05%(n＝9)。结论本研究建立的定性定量方法操作简便,专属性强,准确可靠,可用于痔瘘洗液的质量控制。
목적건립치루세액적질량표준。방법채용박층색보법감별치루세액중적대황、황백,채용고효액상색보법측정해세액중록원산적함량。색보주：Agilent Ecilipse XDB-C18(4.6 mm×250 mm,5μm)；류동상：을정-0.4%린산용액(10∶90)；류속：0.80 mL/min；주온：실온；검측파장：327 nm。결과박층감별방법전속성강,중복성호,공백대조무간우。록원산재2.442～122.1μg범위내정량호적선성관계(r＝1.0000),평균가양회수솔위100.30%, RSD＝1.05%(n＝9)。결론본연구건립적정성정량방법조작간편,전속성강,준학가고,가용우치루세액적질량공제。
Objective To establish the quality standard of Zhilou Lotion. Methods TLC was adopted to identify Radix et Rhizoma Rhei and Cortex Phellodendri. HPLC was adopted to determine the content of chlorogenic acid in Zhilou Lotion. The chromatography column was Agilent Ecilipse XDB-C18 (4.6 mm× 250 mm, 5 μm), the mobile phase was acetonitrile-0.4% phosphoric acid solution (10∶90), the flow rate was 0.80 mL/min, the temperature of column was room temperature, and the detection wavelength was 327 nm. Results The method for identification had good specificity and repeatability. There was no interference in blank control. Chlorogenic acid had good linearity in the range of 2.442-122.1 μg (r=1.000 0). The average recovery rate was 100.30% and RSD was 1.05% (n=9). Conclusion The qualitative and quantitative method is simple, specific, accurate and reliable, and can be used for quality control of Zhilou Lotion.