CAJ | 학술논문

目的采用反相高效液相色谱法测定制萎扶胃丸中隐丹参酮和丹参酮ⅡA 的含量。方法采用十八烷基键合硅胶柱分离隐丹参酮和丹参酮ⅡA,以乙腈-0.2%磷酸(60∶40)为流动相,流速为1.0 mL/min,检测波长262 nm。结果隐丹参酮和丹参酮ⅡA 分别在0.0814～0.4072μg(r＝0.9997)、0.1636～0.818μg(r＝0.9995)范围内呈线性关系,平均回收率分别为99.70%(RSD＝1.37%)、99.22%(RSD＝0.94%)。结论本法简便、快速,可用于该产品的质量控制。
목적채용반상고효액상색보법측정제위부위환중은단삼동화단삼동ⅡA 적함량。방법채용십팔완기건합규효주분리은단삼동화단삼동ⅡA,이을정-0.2%린산(60∶40)위류동상,류속위1.0 mL/min,검측파장262 nm。결과은단삼동화단삼동ⅡA 분별재0.0814～0.4072μg(r＝0.9997)、0.1636～0.818μg(r＝0.9995)범위내정선성관계,평균회수솔분별위99.70%(RSD＝1.37%)、99.22%(RSD＝0.94%)。결론본법간편、쾌속,가용우해산품적질량공제。
Objective To establish the method for determining the content of cryptotanshinone and tanshinone ⅡA in Zhiwei Fuwei Wan. Methods The determination of cryptotanshinone and tanshinone ⅡA was performed on Symmetry C18 column with mobile phase consisted of acetonitrile-0.2%phosphate (60∶40) at flow rate of 1.0 mL/min. The detection wavelength was set at 262 nm. Results The linear ranges of cryptotanshinone and tanshinone ⅡA were 0.081 4-0.407 2 μg (r=0.999 7) and 0.163 6-0.81 8 μg (r=0.999 5) respectively, the average recoveries were 99.70% (RSD=1.37%) and 99.22% (RSD=0.94%) respectively. Conclusion The method is simple and rapid for the quality control of the product.