CAJ | 학술논문

目的初步探讨多种模式肝损伤后Hedgehog信号通路的表达及其变化，为其天然植物阻断剂和中药新药开发及中药治疗肝病提供依据。方法雄性Fisher344大鼠140只适应性喂养1周后，随机分为药物组、手术组、干细胞增生组与正常对照组。按 Gordon’s 方案分次腹腔注射倒千里光碱或对照剂，手术组和干细胞增生组择期行部分肝切除术诱导急性肝损伤与干细胞增殖，药物组、正常对照组予假手术。实时荧光定量PCR、real-time PCR及免疫组化等方法检测原代肝细胞及组织切片中Hedgehog信号通路相关成分在不同时间点的表达。结果多种因素所致肝损伤都可引起Hedgehog信号通路的异常激活，相关成分IHH、PTCH、Smo、Gli1、Gli2、Gli3 mRNA动态表达(SHH阴性)，信号分子IHH及通路激活标志(PTCH、Gli1)3个指标在时间与处理组之间存在交互效应(均P＜0.001)。免疫组化证实PTCH在蛋白水平表达，且不同损伤模式下表达持续的时间不同。结论 Hedgehog信号通路在各种肝损伤后异常激活，可能是参与调控肝损伤后反应的最重要的信号通路之一。
목적초보탐토다충모식간손상후Hedgehog신호통로적표체급기변화，위기천연식물조단제화중약신약개발급중약치료간병제공의거。방법웅성Fisher344대서140지괄응성위양1주후，수궤분위약물조、수술조、간세포증생조여정상대조조。안 Gordon’s 방안분차복강주사도천리광감혹대조제，수술조화간세포증생조택기행부분간절제술유도급성간손상여간세포증식，약물조、정상대조조여가수술。실시형광정량PCR、real-time PCR급면역조화등방법검측원대간세포급조직절편중Hedgehog신호통로상관성분재불동시간점적표체。결과다충인소소치간손상도가인기Hedgehog신호통로적이상격활，상관성분IHH、PTCH、Smo、Gli1、Gli2、Gli3 mRNA동태표체(SHH음성)，신호분자IHH급통로격활표지(PTCH、Gli1)3개지표재시간여처리조지간존재교호효응(균P＜0.001)。면역조화증실PTCH재단백수평표체，차불동손상모식하표체지속적시간불동。결론 Hedgehog신호통로재각충간손상후이상격활，가능시삼여조공간손상후반응적최중요적신호통로지일。
Objective To preliminarily explore the expresssion of the Hedgehog signaling pathway (HH) after different liver trauma, and provide basis for the blockaded effection of HH and the potentiality of new traditional Chinese medicine exploition. Methods After adaptive breeding for one week, a cohort of 140 male SPF Fisher 344 rats were randomly divided into Retrorsine injection (R) indicated the toxical of chemical damage of liver, partial hepatectomy (PH) indicated the acute liver trauma, retrorsine/partial hepatectomy (R/PH) for the hepatic stem cells regeneration, and normal control (N) groups. The administering of retrorsine or placebo, and PH or sham operation were performed as Gordon’s matheds. RT-PCR, real-time PCR and immunohistochemistry were performed to detect the expression of the constituents related to HH after liver damage. Results RT-PCR demonstrated that the mRNA expression of IHH, PTCH, Smo, Gli1, Gli2, Gli3 were all activated in primary liver cells after different kinds of liver damage. The results of real-time PCR analysis indicated an interaction effect existing between the treatment and the time in 3 Hedgehog-related constituents (signaling molecule IHH, indicators of the HH activation Ptc, Gli1) (all P<0.001). The protein expression of PTCH in liver tissue section was confirmed by immunohistochemistry, which differed in the duration in PH and R/PH groups. Conclusion The HH was abnormally activated during the different liver trauma. It’s maybe an important signaling pathway during the liver’s reaction after damage.