CAJ | 학술논문

目的观察吴茱萸汁炮制对黄连抗炎药效及黄连苦寒药性的影响。方法抗炎实验动物模型采用巴豆油致小鼠耳肿胀法。小鼠随机分为对照组、地塞米松组、生黄连组、疏毛萸连组、萸黄连组和石虎萸连组。黄连各组均口服给药1次,给药剂量为6 g/kg,对照组给与同体积蒸馏水,地塞米松组予地塞米松30 mg/kg腹腔注射,观察各组小鼠耳片肿胀程度。药性实验以大鼠为实验动物,分为对照组、生黄连组和3个黄连炮制品组。给药剂量均为3 g/kg,对照组给与同体积蒸馏水。连续口服给药14 d后测定各组大鼠体质量、食量、饮水量、体温、心率、血黏度,并采用放免法检测血清和血浆中促甲状腺素(TSH)、白细胞介素-2(IL-2)、白细胞介素-8(IL-8)含量。结果黄连原药材和不同炮制品均能减轻小鼠耳肿胀程度。黄连各组给药14 d后,大鼠体质量、饮食量、饮水量与对照组比较均有不同程度下降。与对照组比较,疏毛萸连组血黏度升高,IL-2降低(P＜0.05)；生黄连组TSH降低(P＜0.05)。结论不同吴茱萸汁炮制对黄连抗炎药效无明显影响,可在一定程度上减轻黄连的寒性。
목적관찰오수유즙포제대황련항염약효급황련고한약성적영향。방법항염실험동물모형채용파두유치소서이종창법。소서수궤분위대조조、지새미송조、생황련조、소모유련조、유황련조화석호유련조。황련각조균구복급약1차,급약제량위6 g/kg,대조조급여동체적증류수,지새미송조여지새미송30 mg/kg복강주사,관찰각조소서이편종창정도。약성실험이대서위실험동물,분위대조조、생황련조화3개황련포제품조。급약제량균위3 g/kg,대조조급여동체적증류수。련속구복급약14 d후측정각조대서체질량、식량、음수량、체온、심솔、혈점도,병채용방면법검측혈청화혈장중촉갑상선소(TSH)、백세포개소-2(IL-2)、백세포개소-8(IL-8)함량。결과황련원약재화불동포제품균능감경소서이종창정도。황련각조급약14 d후,대서체질량、음식량、음수량여대조조비교균유불동정도하강。여대조조비교,소모유련조혈점도승고,IL-2강저(P＜0.05)；생황련조TSH강저(P＜0.05)。결론불동오수유즙포제대황련항염약효무명현영향,가재일정정도상감경황련적한성。
Objective To explore the influence on anti-inflammatory efficacy and bitter cold properties of Coptis processed by Evodia juice. Methods Auricle swelling model induced by croton oil in mice was used in the anti-inflammatory efficacy experiment. The mice were assigned randomly into distilled control group, dexamethasone group, crude drug group, 3 processed drug groups that Euodia juice which plant origin was Euodia rutaecarpa (Juss.) var. bodinaieri (Dode) Huang (SMYHL), Evodia rutaecarpa (Juss.) Benth and Euodia rutaecarpa (Juss.) Benth var. officinalis (Dode) Huang, respectively. The crude drug group and the 3 processed drugs groups were orally administrated one time and the dosages were all 6 g/kg. The control group was orallly administrated the same volume water. Dexamethasone group was intraperitoneally injected dexamethasone 30 g/kg. The swelling degree of each group was observed. In the bitter cold properties experiment, rats were assigned randomly into control group, crude drug group and 3 processed drug groups. The crude drug group and the 3 processed drug groups were orally administrated for 14 days and the dosages were all 3 g/kg. The control group was orally administrated the same volume water. The body weight, capacity for eating and drinking, body temperature, heart rate and blood viscosity of rats were measured. Meanwhile, TSH, IL-2, IL-8 in serum and plasma were determined by radioimmunoassay. Results The degree of mouse auricle swelling was reduced by the crude and processed drugs. Fourteen days after medication, the weight, capacity of eating and drinking of rats were declined to different degrees in crude drug group and in processed drugs group as compared with control group. The blood viscosity was increased and IL-2 was decreased in SMYHL group as compared with control group (P<0.05). TSH was decreased in crude drug group as compared with control group (P<0.05). Conclusion There is no obvious change in anti-inflammatory function of Coptis before and after processed. The cold properties of Coptis can be reduced after processed by Evodia Juice.