中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2013年
8期
29-31
,共3页
张广平%朱晓光%孟贺%张思玉%杨海润%叶祖光
張廣平%硃曉光%孟賀%張思玉%楊海潤%葉祖光
장엄평%주효광%맹하%장사옥%양해윤%협조광
四逆汤%配伍减毒%附子%动物模型
四逆湯%配伍減毒%附子%動物模型
사역탕%배오감독%부자%동물모형
Sini Decoction%compatibility for attenuation%Aconiti Carmichaeli Radix%animal model
目的针对四逆汤组方中所含的有毒中药附子,从毒理学角度研究四逆汤组方配伍的合理性。方法通过平行比较附子、附子配伍甘草、附子配伍干姜和四逆汤全方水煎液小鼠急性毒性、大鼠心脏毒性和毒性成分,测定小鼠急性毒性的半数致死剂量(LD50)、大鼠心脏毒性半数中毒剂量(TD50)和水煎液液中乌头碱类生物碱含量,从急性毒性、毒性靶器官和毒性成分3个方面研究四逆汤组方中不同配伍对附子毒性的影响。结果四逆汤组方中附子配伍甘草急性毒性的 LD50和心脏毒性的 TD50高于单用附子与四逆汤全方。而附子配伍干姜对附子的急性毒性的 LD50和心脏毒性的 TD50影响不明显。附子配伍甘草水煎液中主要毒性成分双酯型生物碱含量低于附子配伍干姜。结论四逆汤组方配伍药物中甘草对附子毒性具有减毒作用。
目的針對四逆湯組方中所含的有毒中藥附子,從毒理學角度研究四逆湯組方配伍的閤理性。方法通過平行比較附子、附子配伍甘草、附子配伍榦薑和四逆湯全方水煎液小鼠急性毒性、大鼠心髒毒性和毒性成分,測定小鼠急性毒性的半數緻死劑量(LD50)、大鼠心髒毒性半數中毒劑量(TD50)和水煎液液中烏頭堿類生物堿含量,從急性毒性、毒性靶器官和毒性成分3箇方麵研究四逆湯組方中不同配伍對附子毒性的影響。結果四逆湯組方中附子配伍甘草急性毒性的 LD50和心髒毒性的 TD50高于單用附子與四逆湯全方。而附子配伍榦薑對附子的急性毒性的 LD50和心髒毒性的 TD50影響不明顯。附子配伍甘草水煎液中主要毒性成分雙酯型生物堿含量低于附子配伍榦薑。結論四逆湯組方配伍藥物中甘草對附子毒性具有減毒作用。
목적침대사역탕조방중소함적유독중약부자,종독이학각도연구사역탕조방배오적합이성。방법통과평행비교부자、부자배오감초、부자배오간강화사역탕전방수전액소서급성독성、대서심장독성화독성성분,측정소서급성독성적반수치사제량(LD50)、대서심장독성반수중독제량(TD50)화수전액액중오두감류생물감함량,종급성독성、독성파기관화독성성분3개방면연구사역탕조방중불동배오대부자독성적영향。결과사역탕조방중부자배오감초급성독성적 LD50화심장독성적 TD50고우단용부자여사역탕전방。이부자배오간강대부자적급성독성적 LD50화심장독성적 TD50영향불명현。부자배오감초수전액중주요독성성분쌍지형생물감함량저우부자배오간강。결론사역탕조방배오약물중감초대부자독성구유감독작용。
Objective The toxicological compatibility of Aconiti Carmichaeli Radix, as a toxic Chinese medicinal herb, combined with the other ingredients in Sini Decoction was investigated to elucidate the rationality of the combination of the ingredients in Sini Decoction from toxicological point of view. Methods Three kinds of experiments, acute toxicity in mice, heart toxicity in rats and aconitines level in water extract of Sini Decoction and its ingredients including Aconiti Carmichaeli Radix alone and its combination with licorice or dried ginger were adopted in this study. In the toxicological experiments, LD50 values for the acute toxicity test and TD50 values for the heart toxicity (arrhythemia as a parameter) of Sini Decoction, Aconiti Carmichaeli Radix alone and its combination with licorice or dried ginger were comparatively determined. And levels of individual aconitines of the water extracts from Sini Decoction, Aconiti Carmichaeli Radix alone and its combination with licorice or dried ginger were measured, respectively. Results The LD50 and TD50 of the combination of Aconiti Carmichaeli Radix and licorice in Sini Decoction were found to be higher than Aconiti Carmichaeli Radix alone or Sini Decoction, while the LD50 and TD50 of the combination of Aconiti Carmichaeli Radix and dried ginger appeared to be not different from those of Aconiti Carmichaeli Radix alone. The level of the main toxic compound of the water extracts for the combination of Aconiti Carmichaeli Radix and licorice, and Sini Decoction was lower than that of Aconiti Carmichaeli Radix alone and its combination with dried ginger. Conclusion The combination of Aconiti Carmichaeli Radix and licorice can attenuate the toxicity of Aconiti Carmichaeli Radix.