CAJ | 학술논문

目的完善静脉炎合剂的质量控制标准。方法采用T LC法对静脉炎合剂方中红花、当归、绵萆薢、伸筋草进行定性鉴别；采用HPLC法测定防己中粉防己碱的含量，色谱柱：Agilent Zorbax Extend-C18柱（4．6 mm ×150 mm ，5μm）；流动相：甲醇-0．3％二乙胺（75∶25）；检测波长：282 nm ，柱温：27℃，流速：1．0 mL/min。结果薄层色谱图清晰，阴性对照无干扰；粉防己碱在5．18～259．00μg/m L浓度范围内与峰面积呈良好线性关系， r ＝0．9999（n ＝8），平均加样回收率为99．8％（RSD＝1．91％，n ＝9）。结论本实验建立的质量控制方法简便、准确、重现性好，可用于静脉炎合剂的质量控制。
목적완선정맥염합제적질량공제표준。방법채용T LC법대정맥염합제방중홍화、당귀、면비해、신근초진행정성감별；채용HPLC법측정방기중분방기감적함량，색보주：Agilent Zorbax Extend-C18주（4．6 mm ×150 mm ，5μm）；류동상：갑순-0．3％이을알（75∶25）；검측파장：282 nm ，주온：27℃，류속：1．0 mL/min。결과박층색보도청석，음성대조무간우；분방기감재5．18～259．00μg/m L농도범위내여봉면적정량호선성관계， r ＝0．9999（n ＝8），평균가양회수솔위99．8％（RSD＝1．91％，n ＝9）。결론본실험건립적질량공제방법간편、준학、중현성호，가용우정맥염합제적질량공제。
Objective To improve the quality control standards for phlebitis mixture .Methods TLC was applied to identify flowers carthami ,Angelica sinensis ,Rhizoma Dioscoreae Septemlobae ,and Herba Lyco-podii .HPLC analysis was carried out by using Agilent Zorbax Extend-C18 column with methanol -0 .3%diethylamine (75∶25) as mobile phase .The detection wavelength was 282 mm ,the column temperature was 27℃ and the flow rate 1 .0 mL/min .Results The thin-layer chromatogram was distinct and the nega-tive control had no interference .The linear relation between peak area and concentration of tetrandrine (5 .18-259 .00 μg/mL) were good (r = 0 .999 9 ,n =8) .The average recovery rate was 99 .8% (RSD=1 .91% ,n=9) .Conclusion This method based on the experiment was proved to be simple ,accurate and highly reproducible .It could be used for quality control in the production of phlebitis mixture .