CAJ | 학술논문

目的研究金雀花的薄层色谱鉴别特征，建立HPLC法测定黄酮类化合物的方法。方法采用薄层色谱法对金雀花中槲皮素和山萘酚进行定性鉴别，槲皮素以甲苯-乙酸乙酯-甲酸＝5∶2∶1为展开剂，山萘素以甲苯-乙酸乙酯-甲酸＝5∶4∶1为展开剂；采用色谱柱：Shim-pack VP-ODS（150 mm ×4．6 mm），以甲醇-0．4％磷酸溶液（50∶50）为流动相，流速为1 mL/min ，检测波长为360 nm ，柱温为25℃。结果在薄层色谱中能检测到槲皮素和山萘酚，槲皮素、山萘素、异鼠李素分别在0．01～0．1、0．0025～0．025、0．0025～0．025 mg/mL范围内线性良好，其平均回收率分别为100．7％、100．4％、101．7％。RSD分别为1．3％、1．4％和1．9％。样品黄酮类化合物的平均含量为1．25％。结论该方法简便、快速、准确、重复性好，可用于测定金雀花中黄酮类化合物的含量。
목적연구금작화적박층색보감별특정，건립HPLC법측정황동류화합물적방법。방법채용박층색보법대금작화중곡피소화산내분진행정성감별，곡피소이갑분-을산을지-갑산＝5∶2∶1위전개제，산내소이갑분-을산을지-갑산＝5∶4∶1위전개제；채용색보주：Shim-pack VP-ODS（150 mm ×4．6 mm），이갑순-0．4％린산용액（50∶50）위류동상，류속위1 mL/min ，검측파장위360 nm ，주온위25℃。결과재박층색보중능검측도곡피소화산내분，곡피소、산내소、이서리소분별재0．01～0．1、0．0025～0．025、0．0025～0．025 mg/mL범위내선성량호，기평균회수솔분별위100．7％、100．4％、101．7％。RSD분별위1．3％、1．4％화1．9％。양품황동류화합물적평균함량위1．25％。결론해방법간편、쾌속、준학、중복성호，가용우측정금작화중황동류화합물적함량。
Objective To establish a HPLC method for the determination of flavonoids content in caragana altaica Kom .Pojark by the studies on TLC identification characteristics of Caragana flowers in Aletai . Methods Quercetin and kaempferol were identified by TLC ,The HPLC analysis was performed on a Sun-fire C18 (150 mm × 4 .6 mm ,5 μm)column .a mixture of acetonitrile -0 .4% phosphoric acid aqueous solu-tion (50∶50) was used as the mobile phase with the flow rate at 1 mL/min .The detection wavelength was set at 360 nm .The column temperature was set at 25℃ .Results Quercetin and kaempferol could be de-tected by TLC quercetin ,kaempferol and isorhamnetin showed good linearity in the ranges of 0 .01 -0 .10 mg/mL ,0 .0025-0 .025 mg/mL and 0 .0025-0 .025 mg/mL .The average recoveries were 100 .7% , 100 .4% ,101 .7% .RSD1 .3% ,1 .4% and 1 .9% .Samples of flavonoid content averaged 1 .3% .Conclu-sion T he method is simple ,rapid ,accurate and reproducible and it can be used for the determination of the caragana altaica Kom .Pojark in the flavonoids content .