CAJ | 학술논문

通过对武威市凉州区人参果感病植株所表现出来的花叶、皱缩、植株矮小等症状观察，初步判断可能感染了马铃薯M病毒（PVM）。结合分子生物学手段，提取感病人参果叶片总RNA，采用RT-PCR方法扩增到一段长300 bp的基因序列，连接至PMD19-T载体并测序。测序结果同GeneBank中PVM CP基因序列[GI：361071296]同源性达到了98.97%，说明成功克隆到了PVM基因片段，即供试人参果确实感染了PVM病毒。
통과대무위시량주구인삼과감병식주소표현출래적화협、추축、식주왜소등증상관찰，초보판단가능감염료마령서M병독（PVM）。결합분자생물학수단，제취감병인삼과협편총RNA，채용RT-PCR방법확증도일단장300 bp적기인서렬，련접지PMD19-T재체병측서。측서결과동GeneBank중PVM CP기인서렬[GI：361071296]동원성체도료98.97%，설명성공극륭도료PVM기인편단，즉공시인삼과학실감염료PVM병독。
The mosaic,wrinkled,small plant and other symptoms of susceptible plants of Liangzhou ginseng fruit were observed,the auditor should make a pre-judgment that may be infected with potato virus M (PVM). Experiments combined with molecular biology methods and extract leaves total RNA of the patient feeling of ginseng fruit,it was amplified by RT-PCR method to a long 300 bp gene sequences and connected to PMD19-T vector and sequenced. The Sequencing results showed that homology reached 98.97%with the GeneBank PVM CP gene sequence [GI:361071296],it can explain gene fragment was cloned into the PVM successful,that the test does ginseng fruit infected with PVM virus.