中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
9期
3910-3913
,共4页
姚娇%葛书霞%丁朝霞%陈爱平
姚嬌%葛書霞%丁朝霞%陳愛平
요교%갈서하%정조하%진애평
卵巢肿瘤%细胞外信号调节MAP激酶类%细胞凋亡
卵巢腫瘤%細胞外信號調節MAP激酶類%細胞凋亡
란소종류%세포외신호조절MAP격매류%세포조망
Ovarian neoplasms%Extracellular signal-regulated MAP kinases%Apoptosis
目的研究细胞外信号调节激酶ERK1/2在卵巢癌细胞SKOV3中的表达及其与细胞相关凋亡蛋白的关系。方法用MTT检测17β-E2对细胞的增殖情况，用MEK1/2通路抑制剂U0126在不同浓度、不同时间处理细胞后，RT-PCR检测细胞ERK1 mRNA、ERK2 mRNA、caspase-3 mRNA的表达。结果浓度为10-6 mol/L 17β-E2作用1 h可显著促进细胞增殖[（5.7±0.23）％]，与对照组[（3.5±0.45）％]相比，P＝0.000。 ERK1/2在卵巢癌中高表达，随着U0126处理细胞浓度的增加及时间的延长， ERK1的表达逐渐降低，与对照组相比，P值分别为0.176、0.009、0.002、0.000；ERK2的表达逐渐降低，与对照组相比，P值分别为0.010、0.000、0.000、0.000；caspase-3的表达逐渐增加，与对照组相比， P值分别为1.000、0.290、0.090、0.003。结论 ERK1/2与卵巢癌的发生发展有关，抑制ERK1/2的表达可抑制细胞增殖，增加细胞凋亡。
目的研究細胞外信號調節激酶ERK1/2在卵巢癌細胞SKOV3中的錶達及其與細胞相關凋亡蛋白的關繫。方法用MTT檢測17β-E2對細胞的增殖情況，用MEK1/2通路抑製劑U0126在不同濃度、不同時間處理細胞後，RT-PCR檢測細胞ERK1 mRNA、ERK2 mRNA、caspase-3 mRNA的錶達。結果濃度為10-6 mol/L 17β-E2作用1 h可顯著促進細胞增殖[（5.7±0.23）％]，與對照組[（3.5±0.45）％]相比，P＝0.000。 ERK1/2在卵巢癌中高錶達，隨著U0126處理細胞濃度的增加及時間的延長， ERK1的錶達逐漸降低，與對照組相比，P值分彆為0.176、0.009、0.002、0.000；ERK2的錶達逐漸降低，與對照組相比，P值分彆為0.010、0.000、0.000、0.000；caspase-3的錶達逐漸增加，與對照組相比， P值分彆為1.000、0.290、0.090、0.003。結論 ERK1/2與卵巢癌的髮生髮展有關，抑製ERK1/2的錶達可抑製細胞增殖，增加細胞凋亡。
목적연구세포외신호조절격매ERK1/2재란소암세포SKOV3중적표체급기여세포상관조망단백적관계。방법용MTT검측17β-E2대세포적증식정황，용MEK1/2통로억제제U0126재불동농도、불동시간처리세포후，RT-PCR검측세포ERK1 mRNA、ERK2 mRNA、caspase-3 mRNA적표체。결과농도위10-6 mol/L 17β-E2작용1 h가현저촉진세포증식[（5.7±0.23）％]，여대조조[（3.5±0.45）％]상비，P＝0.000。 ERK1/2재란소암중고표체，수착U0126처리세포농도적증가급시간적연장， ERK1적표체축점강저，여대조조상비，P치분별위0.176、0.009、0.002、0.000；ERK2적표체축점강저，여대조조상비，P치분별위0.010、0.000、0.000、0.000；caspase-3적표체축점증가，여대조조상비， P치분별위1.000、0.290、0.090、0.003。결론 ERK1/2여란소암적발생발전유관，억제ERK1/2적표체가억제세포증식，증가세포조망。
Objective To investigate the expression of extracellular signa 1-regulated kinase1/2(ERK1/2) in ovarian carcinoma cell and its correlation with apoptosis .Methods The proliferation was assayed by MTT when the cells were exposed to 17β-E2 .After the cell was treated with U 0126 which is MEK suppressor ,the expression of caspase-3,ERK1,ERK2 mRNA in ovarian carcinoma cell were detected by RT-PCR in different concentration and different time.Results The cell proliferative rate was significantly higher [(5.7 ±0.23)%]after stimulation with 17β-E2 at 1 ×10 -6 mol/L for 60 min than control [ ( 3.5 ±0.45 )%] ( P=0.000 ) .ERK1/2 was highly expressed , after the cell were treated with U0126,along with increasing of time and concentration ,the expression of ERK1 was increasingly reduction,compared to control group,P =0.176,0.009,0.002,0.000;the expression of ERK2 was increasingly reduction too , compared to control group , P =0.010 , 0.000 , 0.000 , 0.000;while the expression of caspase-3 were increasingly highly than control , P =1.000 , 0.290 , 0.090 , 0.003 .Conclusion ERK1/2 is participate in the occurrence and development of ovarian cancer .When ERK1/2 expression is inhibited , cell proliferation is inhibited ,while cell apoptosis is increased .
