中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
9期
3910-3913
,共4页
姚娇%葛书霞%丁朝霞%陈爱平
姚嬌%葛書霞%丁朝霞%陳愛平
요교%갈서하%정조하%진애평
卵巢肿瘤%细胞外信号调节MAP激酶类%细胞凋亡
卵巢腫瘤%細胞外信號調節MAP激酶類%細胞凋亡
란소종류%세포외신호조절MAP격매류%세포조망
Ovarian neoplasms%Extracellular signal-regulated MAP kinases%Apoptosis
目的研究细胞外信号调节激酶ERK1/2在卵巢癌细胞SKOV3中的表达及其与细胞相关凋亡蛋白的关系。方法用MTT检测17β-E2对细胞的增殖情况,用MEK1/2通路抑制剂U0126在不同浓度、不同时间处理细胞后,RT-PCR检测细胞ERK1 mRNA、ERK2 mRNA、caspase-3 mRNA的表达。结果浓度为10-6 mol/L 17β-E2作用1 h可显著促进细胞增殖[(5.7±0.23)%],与对照组[(3.5±0.45)%]相比,P=0.000。 ERK1/2在卵巢癌中高表达,随着U0126处理细胞浓度的增加及时间的延长, ERK1的表达逐渐降低,与对照组相比,P值分别为0.176、0.009、0.002、0.000;ERK2的表达逐渐降低,与对照组相比,P值分别为0.010、0.000、0.000、0.000;caspase-3的表达逐渐增加,与对照组相比, P值分别为1.000、0.290、0.090、0.003。结论 ERK1/2与卵巢癌的发生发展有关,抑制ERK1/2的表达可抑制细胞增殖,增加细胞凋亡。
目的研究細胞外信號調節激酶ERK1/2在卵巢癌細胞SKOV3中的錶達及其與細胞相關凋亡蛋白的關繫。方法用MTT檢測17β-E2對細胞的增殖情況,用MEK1/2通路抑製劑U0126在不同濃度、不同時間處理細胞後,RT-PCR檢測細胞ERK1 mRNA、ERK2 mRNA、caspase-3 mRNA的錶達。結果濃度為10-6 mol/L 17β-E2作用1 h可顯著促進細胞增殖[(5.7±0.23)%],與對照組[(3.5±0.45)%]相比,P=0.000。 ERK1/2在卵巢癌中高錶達,隨著U0126處理細胞濃度的增加及時間的延長, ERK1的錶達逐漸降低,與對照組相比,P值分彆為0.176、0.009、0.002、0.000;ERK2的錶達逐漸降低,與對照組相比,P值分彆為0.010、0.000、0.000、0.000;caspase-3的錶達逐漸增加,與對照組相比, P值分彆為1.000、0.290、0.090、0.003。結論 ERK1/2與卵巢癌的髮生髮展有關,抑製ERK1/2的錶達可抑製細胞增殖,增加細胞凋亡。
목적연구세포외신호조절격매ERK1/2재란소암세포SKOV3중적표체급기여세포상관조망단백적관계。방법용MTT검측17β-E2대세포적증식정황,용MEK1/2통로억제제U0126재불동농도、불동시간처리세포후,RT-PCR검측세포ERK1 mRNA、ERK2 mRNA、caspase-3 mRNA적표체。결과농도위10-6 mol/L 17β-E2작용1 h가현저촉진세포증식[(5.7±0.23)%],여대조조[(3.5±0.45)%]상비,P=0.000。 ERK1/2재란소암중고표체,수착U0126처리세포농도적증가급시간적연장, ERK1적표체축점강저,여대조조상비,P치분별위0.176、0.009、0.002、0.000;ERK2적표체축점강저,여대조조상비,P치분별위0.010、0.000、0.000、0.000;caspase-3적표체축점증가,여대조조상비, P치분별위1.000、0.290、0.090、0.003。결론 ERK1/2여란소암적발생발전유관,억제ERK1/2적표체가억제세포증식,증가세포조망。
Objective To investigate the expression of extracellular signa 1-regulated kinase1/2(ERK1/2) in ovarian carcinoma cell and its correlation with apoptosis .Methods The proliferation was assayed by MTT when the cells were exposed to 17β-E2 .After the cell was treated with U 0126 which is MEK suppressor ,the expression of caspase-3,ERK1,ERK2 mRNA in ovarian carcinoma cell were detected by RT-PCR in different concentration and different time.Results The cell proliferative rate was significantly higher [(5.7 ±0.23)%]after stimulation with 17β-E2 at 1 ×10 -6 mol/L for 60 min than control [ ( 3.5 ±0.45 )%] ( P=0.000 ) .ERK1/2 was highly expressed , after the cell were treated with U0126,along with increasing of time and concentration ,the expression of ERK1 was increasingly reduction,compared to control group,P =0.176,0.009,0.002,0.000;the expression of ERK2 was increasingly reduction too , compared to control group , P =0.010 , 0.000 , 0.000 , 0.000;while the expression of caspase-3 were increasingly highly than control , P =1.000 , 0.290 , 0.090 , 0.003 .Conclusion ERK1/2 is participate in the occurrence and development of ovarian cancer .When ERK1/2 expression is inhibited , cell proliferation is inhibited ,while cell apoptosis is increased .