计量学报
計量學報
계량학보
ACTA METROLOGICA SINICA
2014年
3期
291-295
,共5页
叶含笑%金红婷%袁昕%江依法
葉含笑%金紅婷%袁昕%江依法
협함소%금홍정%원흔%강의법
计量学%骨组织形态%苏木精-伊红染色法%视觉模型%RGB颜色模型%欧氏距离%图像分割
計量學%骨組織形態%囌木精-伊紅染色法%視覺模型%RGB顏色模型%歐氏距離%圖像分割
계량학%골조직형태%소목정-이홍염색법%시각모형%RGB안색모형%구씨거리%도상분할
Metrology%Bone tissue morphology%Hematoxylin-eosin staining%Visual model%RGB color model%Euclidean distance%Image segmentation
为了提取HE染色骨组织切片的骨小梁,实现骨小梁面积定量检测,以极坐标扩散方式创建视觉模型,划分骨小梁与骨细胞、骨皮质的边界。提取背景某一像素的RGB值,把病理切片所有像素的RGB值对背景像素求取三维欧式距离,获得阈值,对图像二值化分割,通过区域填充获得骨小梁。结果表明视觉模型能较好地获得感兴趣区域的边界,计量的骨小梁面积精度高于常规软件,检测结果鲁棒性相对于常规软件提高了95豫以上,人机交互少,降低了检测的盲目性,提高了检测速度。
為瞭提取HE染色骨組織切片的骨小樑,實現骨小樑麵積定量檢測,以極坐標擴散方式創建視覺模型,劃分骨小樑與骨細胞、骨皮質的邊界。提取揹景某一像素的RGB值,把病理切片所有像素的RGB值對揹景像素求取三維歐式距離,穫得閾值,對圖像二值化分割,通過區域填充穫得骨小樑。結果錶明視覺模型能較好地穫得感興趣區域的邊界,計量的骨小樑麵積精度高于常規軟件,檢測結果魯棒性相對于常規軟件提高瞭95豫以上,人機交互少,降低瞭檢測的盲目性,提高瞭檢測速度。
위료제취HE염색골조직절편적골소량,실현골소량면적정량검측,이겁좌표확산방식창건시각모형,화분골소량여골세포、골피질적변계。제취배경모일상소적RGB치,파병리절편소유상소적RGB치대배경상소구취삼유구식거리,획득역치,대도상이치화분할,통과구역전충획득골소량。결과표명시각모형능교호지획득감흥취구역적변계,계량적골소량면적정도고우상규연건,검측결과로봉성상대우상규연건제고료95예이상,인궤교호소,강저료검측적맹목성,제고료검측속도。
To extract trabecular bone of hematoxylin-eosin staining staining bone tissue pathological slices,for quantitative detection of trabecular bone area,create a polar diffusion model,to let computer extract any hematoxylin-eosin staining dyeing bone slice trabecular bone,divide the boundary of the trabecular bone with bone cells and bone cortex. Extract a pixel’s RGB values of background,use RGB color value of all pixels with the background pixels to calculate 3d euclidean distance,get threshold,to achieve image binarization segmentation,by area filling get trabecular bone. Results show that the visual model can get the boundary of the region of interest well. Measurement accuracy and speed are significantly improved. Results of measurement relative to the conventional software the robustness improve over 95%, reduce human-computer interaction,to reduce the blindness of testing.