CAJ | 학술논문

目的探讨靶向Survivin的siRNA对卵巢癌细胞ov-1063细胞生长、凋亡的影响。方法用lipofectamineTM2000使Survivin的siRNA真核表达载体转染至卵巢癌ov-1063细胞，MTT法测对ov-1063细胞生长、增殖的影响，膜联蛋白染色、流式细胞仪检测ov-1063细胞凋亡情况。结果靶向Survivin的特异性siRNA能高效率抑制ov-1063细胞的Survivin基因表达。结论利用RNAi技术沉默Survivin基因表达可以抑制 ov-1063的表达，诱导其自发凋亡，此项技术在卵巢癌的基因中具有一定价值。
목적탐토파향Survivin적siRNA대란소암세포ov-1063세포생장、조망적영향。방법용lipofectamineTM2000사Survivin적siRNA진핵표체재체전염지란소암ov-1063세포，MTT법측대ov-1063세포생장、증식적영향，막련단백염색、류식세포의검측ov-1063세포조망정황。결과파향Survivin적특이성siRNA능고효솔억제ov-1063세포적Survivin기인표체。결론이용RNAi기술침묵Survivin기인표체가이억제 ov-1063적표체，유도기자발조망，차항기술재란소암적기인중구유일정개치。
Objective To investigate the ef ect of Survivin siRNA target on the growth, apoptosis in human ovarian cancer OV-1063 cel s. Methods lipofectamineTM2000 siRNA eukaryotic expression vector was transfected into ovarian cancer Ov-1063 cel s was detected by MTT Survivin, influence on the growth, proliferation of OV-1063 cel s, annexin staining, apoptosis of the OV-1063 cel s was detected by flow cytometry. Results the targeting specificity of siRNA Survivin can ef iciently inhibit OV-1063 cel Survivin gene expression. Conclusion the expression of using RNAi technology to silence Survivin gene expression can inhibit the spontaneous apoptosis induced by OV-1063, forget, this technique has certain value in ovarian cancer gene.