CAJ | 학술논문

目的：通过观察转录因子FoxO3a在小鼠牙胚发育中的表达特点及分布情况，探讨其在小鼠牙胚发育过程中的作用。方法取出生0 d和3 d的昆明小鼠各3只，处死解剖下颌骨，放于新鲜配置的10％中性甲醛溶液中固定过夜，制备小鼠牙胚0d和3d的组织标本，常规石蜡包埋，近远中向5μm连续切片，采用免疫组化方法观察检测FoxO3 a在小鼠不同时期的牙胚表达情况和分布情况，并对结果进行分析。结果 FoxO3 a在小鼠不同时期的牙胚发育中的分布存在差异，FoxO3a在0d小鼠牙胚中，成牙本质细胞成阴性表达，在3d小鼠牙胚中成牙本质细胞成阳性表达。结论观察FoxO3a在小鼠牙胚发育过程中的表达以及表达的变化，提示FoxO3a可能参与牙本质的形成和矿化。
목적：통과관찰전록인자FoxO3a재소서아배발육중적표체특점급분포정황，탐토기재소서아배발육과정중적작용。방법취출생0 d화3 d적곤명소서각3지，처사해부하합골，방우신선배치적10％중성갑철용액중고정과야，제비소서아배0d화3d적조직표본，상규석사포매，근원중향5μm련속절편，채용면역조화방법관찰검측FoxO3 a재소서불동시기적아배표체정황화분포정황，병대결과진행분석。결과 FoxO3 a재소서불동시기적아배발육중적분포존재차이，FoxO3a재0d소서아배중，성아본질세포성음성표체，재3d소서아배중성아본질세포성양성표체。결론관찰FoxO3a재소서아배발육과정중적표체이급표체적변화，제시FoxO3a가능삼여아본질적형성화광화。
Objective To explore role of the expression and distribution of transcription factor FoxO 3 a in the development of mouse tooth germ .Methods Three new born KunMing mice and three 3 days old KunMing mice were chosen ,and their mandibles were dissected ,fixed overnight in the 10%neutral formaldehyde solution .Tissue sam-ples of mouse tooth germ were made ,embedded in paraffin ,and prepared to be close to 5μm serial sections .The expres-sion and distribution of transcription factor FoxO 3a in the development of mouse tooth germ at different stages were ob-served by immunohistochemistry methods and analysis was took .Results There were differences among distribution of FoxO3 a in the development of mouse tooth germ at different stages Negative expression of odontoblast was found in new born nice,and the positive expression was in 3 days mice.Conclusion FoxO3a may be involved in the formation and mineralization of the dentin ,according to expression of FoxO3a in the development of mouse tooth germ .