广西医学
廣西醫學
엄서의학
GUANGXI MEDICAL JOURNAL
2013年
10期
1273-1274,1287
,共3页
吴峰萍%张劼%罗佐杰%文静%梁杏欢
吳峰萍%張劼%囉佐傑%文靜%樑杏歡
오봉평%장할%라좌걸%문정%량행환
肾上腺皮质癌%羟喜树碱%人肾上腺皮质癌SW-13细胞%台盼蓝拒染法
腎上腺皮質癌%羥喜樹堿%人腎上腺皮質癌SW-13細胞%檯盼藍拒染法
신상선피질암%간희수감%인신상선피질암SW-13세포%태반람거염법
Adrenoeortical carcinoma%Hydroxycamptothecine%Human adrenoeortical carcinoma cell line SW-13%Trypan blue exclusion staining
目的观察羟喜树碱对人肾上腺皮质癌SW-13细胞生长增殖的影响。方法体外培养人肾上腺皮质癌SW-13细胞,随机分为干预组和对照组,干预组加入羟喜树碱使其终浓度为0.8μg/ml,对照组不加羟喜树碱,分别培养48 h后,采用台盼蓝拒染法观察羟喜树碱对SW-13细胞生长增殖的影响。结果光镜下可见干预组SW-13细胞数减少,死亡细胞数较多,干预组活细胞率明显低于对照组( P<0.05)。结论羟喜树碱可能具有抑制人肾上腺皮质癌SW-13细胞增殖并促进其死亡的作用。
目的觀察羥喜樹堿對人腎上腺皮質癌SW-13細胞生長增殖的影響。方法體外培養人腎上腺皮質癌SW-13細胞,隨機分為榦預組和對照組,榦預組加入羥喜樹堿使其終濃度為0.8μg/ml,對照組不加羥喜樹堿,分彆培養48 h後,採用檯盼藍拒染法觀察羥喜樹堿對SW-13細胞生長增殖的影響。結果光鏡下可見榦預組SW-13細胞數減少,死亡細胞數較多,榦預組活細胞率明顯低于對照組( P<0.05)。結論羥喜樹堿可能具有抑製人腎上腺皮質癌SW-13細胞增殖併促進其死亡的作用。
목적관찰간희수감대인신상선피질암SW-13세포생장증식적영향。방법체외배양인신상선피질암SW-13세포,수궤분위간예조화대조조,간예조가입간희수감사기종농도위0.8μg/ml,대조조불가간희수감,분별배양48 h후,채용태반람거염법관찰간희수감대SW-13세포생장증식적영향。결과광경하가견간예조SW-13세포수감소,사망세포수교다,간예조활세포솔명현저우대조조( P<0.05)。결론간희수감가능구유억제인신상선피질암SW-13세포증식병촉진기사망적작용。
Objective To observe the effect of hydroxycamptothecine ( HCPT) on the growth and proliferation of human adrenoeortical carcinoma cell line SW-13.Methods Human adrenoeortical carcinoma cells SW-13 cultured in vitro were randomly divided into two groups ,the 0.8ug/ml HCPT intervention group and negative control group . SW-13 cells were exposed to hydroxycamptothecine for 48 hours.The effect of hydroxycamptothecine on the growth and proliferation of human adrenoeortical carcinoma cell line SW-13 was observed by trypan blue exclusion staining . Results The number of SW-13 cells decreased in the intervention group by light microscope ,the number of dead cells increased significantly .The live cells in the intervention group were more than those in the control group (P<0.05). Conclusion Hydroxycamptothecine may suppress the proliferation of human adrenoeortical carcinoma cell line SW -13 and promote the death of SW-13 cells.