CAJ | 학술논문

试验将300羽罗曼蛋鸡随机分成3组，每组5个重复，每个重复20只鸡。1组为对照组，饲喂基础蛋鸡日粮，2组和3组为试验组，在基础日粮中分别添加0.2 mg/kg富硒酵母和亚硒酸钠。试验期为35 d。于试验第35天采取组织样品测定硒含量、GPX活性和肝脏GPX-4 mRNA表达水平。结果表明：富硒酵母组和亚硒酸钠组蛋鸡肝脏、肾脏、胸肌硒含量，肝脏、肾脏GPX活性以及肝脏GPX-4 mRNA表达水平均显著或极显著高于对照组（P＜0.05或P＜0.01）；富硒酵母组蛋鸡的胸肌硒含量，肝脏、肾脏GPX活性均显著高于亚硒酸钠组（P＜0.05）；亚硒酸钠组蛋鸡的肾脏硒含量显著高于富硒酵母组（P＜0.05）；而富硒酵母组和亚硒酸钠组之间肝脏硒含量和肝脏GPX-4 mRNA表达水平无显著差异（P＞0.05）。
시험장300우라만단계수궤분성3조，매조5개중복，매개중복20지계。1조위대조조，사위기출단계일량，2조화3조위시험조，재기출일량중분별첨가0.2 mg/kg부서효모화아서산납。시험기위35 d。우시험제35천채취조직양품측정서함량、GPX활성화간장GPX-4 mRNA표체수평。결과표명：부서효모조화아서산납조단계간장、신장、흉기서함량，간장、신장GPX활성이급간장GPX-4 mRNA표체수평균현저혹겁현저고우대조조（P＜0.05혹P＜0.01）；부서효모조단계적흉기서함량，간장、신장GPX활성균현저고우아서산납조（P＜0.05）；아서산납조단계적신장서함량현저고우부서효모조（P＜0.05）；이부서효모조화아서산납조지간간장서함량화간장GPX-4 mRNA표체수평무현저차이（P＞0.05）。
A total of 300 Roman laying hens were randomly assigned into 3 dietary treatment groups with 5 replicates of 20 each for an 5 weeks study,which were fed basal diet or basal diets supplemented with 0.20 mg/kg Se from Se-en-riched yeast,sodium selenite,respectively.Tissue samples were collected at experiment termination.Tissue Se concentra-tions,tissue glutathione peroxidase(GPX)activities and liver GPX-4 mRNA levels were analyzed.The results showed that:the kidney Se concentrations,breast muscle Se concentrations,liver GPX activities and kidney GPX activities increased significantly of 2 supplemented groups (P < 0.01 or P < 0.05)compared with the control group.The liver GPX activities, kidney GPX activities and breast muscle Se concentrations of Se-enriched yeast group were higher than those of sodium selenite group (P < 0.05).However,the kidney Se concentrations of sodium selenite group were higher than those of Se-enriched yeast group (P<0.05).But there was no significant differences in liver Se concentrations and liver GPX-4 mR-NA levels between Se-enriched yeast group and sodium selenite group.