CAJ | 학술논문

试验旨在探讨用地衣芽孢杆菌益生素替代常规颗粒饲料中的抗生素后对肉鸡生长性能、盲肠微生物、消化道pH值及免疫器官指数的影响。采用2×2析因试验设计，试验因素为蛋白水平（0~21 d：20.8%与20.3%；22~35 d：19.3%与18.8%）和促生长添加剂（抗生素与益生素）。选用1日龄山东地方杂交鸡商品肉仔鸡约24万只，随机分成4个处理，每个处理6个重复，每个重复约1万只。结果表明：地衣芽孢杆菌替代抗生素显著提高了肉鸡后期（22~35 d）的日增重（P<0.05），并有增加后期日采食量、35 d体重及全期日增重的趋势（P<0.1）；肉鸡21 d时回肠、盲肠和直肠的pH值显著降低（P<0.05），显著降低了肉鸡全期盲肠内大肠杆菌的数量和增加了乳酸菌数量（P<0.05），显著增加了21 d的胸腺指数、35 d的胸腺指数和法氏囊指数（P<0.05）；肉鸡生长性能和其他指标在不同蛋白水平之间无显著差异，交互作用不显著。与抗生素相比，地衣芽孢杆菌有增加杂交肉鸡后期生产性能的趋势，可通过调节盲肠微生态、降低前期后段肠道pH值来改善肉鸡肠道环境，能显著提高免疫器官指数。
시험지재탐토용지의아포간균익생소체대상규과립사료중적항생소후대육계생장성능、맹장미생물、소화도pH치급면역기관지수적영향。채용2×2석인시험설계，시험인소위단백수평（0~21 d：20.8%여20.3%；22~35 d：19.3%여18.8%）화촉생장첨가제（항생소여익생소）。선용1일령산동지방잡교계상품육자계약24만지，수궤분성4개처리，매개처리6개중복，매개중복약1만지。결과표명：지의아포간균체대항생소현저제고료육계후기（22~35 d）적일증중（P<0.05），병유증가후기일채식량、35 d체중급전기일증중적추세（P<0.1）；육계21 d시회장、맹장화직장적pH치현저강저（P<0.05），현저강저료육계전기맹장내대장간균적수량화증가료유산균수량（P<0.05），현저증가료21 d적흉선지수、35 d적흉선지수화법씨낭지수（P<0.05）；육계생장성능화기타지표재불동단백수평지간무현저차이，교호작용불현저。여항생소상비，지의아포간균유증가잡교육계후기생산성능적추세，가통과조절맹장미생태、강저전기후단장도pH치래개선육계장도배경，능현저제고면역기관지수。
The study was conducted to investigate the effects of Bacillus licheniformis probiotic as antibiotic substitution on growth performance, intestinal health and immune organ indexes of broilers. Two hundred and forty thousand 1-day-old Shandong local cross broilers were randomly divided into a 2 × 2 factorial design(CP level × Growth promotion) containing 4 dietary treatments consisting of 6 replicates of about 10 000 birds each. Results showed that ADG on 22 to 35 d was significantly increased(P<0.05) in probiotic treatments. There are increasing trends(P<0.1) in ADFI on 22 to 35 d, BW on 35 d, and ADG on 0-35 d. Broilers fed diets containing B. lichenifor-mis had lower pH value in ileum, cecum, and rectal on 21 d(P<0.05), lower E. coli and higher lactic acid bacteria counts(P<0.05). Compared with antibiotic, B. licheniformis significantly im-proved the thymus indexs on 21 d and 35 d and bursa index on 35 d(P<0.05). No significant dif-ferences were observed between H-CP and L-CP treatments. B. licheniformis probiotic as antibi-otic substitution had an increasing trend in growth performance, and significantly improved intesti-nal flora balance and immune organs indexes.