CAJ | 학술논문

黄曲霉 Aspergillus f lavus 是一种广泛存在的致病真菌，早期准确检测黄曲霉并加以控制是减少黄曲霉毒素产生的有效措施。本研究利用真菌通用引物 ITS1/ITS4扩增黄曲霉转录间隔区并进行克隆测序，通过序列比对，设计1对黄曲霉特异性引物 S1/X2，由此建立的 PCR 检测体系对包括黄曲霉在内的5种曲霉菌及其他17种不同的真菌、细菌基因组 DNA 进行扩增，结果只有不同来源的黄曲霉菌株能扩增出334 bp 的特异性条带，而其余参试菌株均无扩增产物，其检测灵敏度在 DNA 水平上可达到280 fg 。该检测体系能从自然发病的花生或玉米组织中扩增到334 bp 的特异片段，实现对黄曲霉的快速可靠检测。
황곡매 Aspergillus f lavus 시일충엄범존재적치병진균，조기준학검측황곡매병가이공제시감소황곡매독소산생적유효조시。본연구이용진균통용인물 ITS1/ITS4확증황곡매전록간격구병진행극륭측서，통과서렬비대，설계1대황곡매특이성인물 S1/X2，유차건립적 PCR 검측체계대포괄황곡매재내적5충곡매균급기타17충불동적진균、세균기인조 DNA 진행확증，결과지유불동래원적황곡매균주능확증출334 bp 적특이성조대，이기여삼시균주균무확증산물，기검측령민도재 DNA 수평상가체도280 fg 。해검측체계능종자연발병적화생혹옥미조직중확증도334 bp 적특이편단，실현대황곡매적쾌속가고검측。
Aspergillus flavus is a ubiquitous fungal pathogen in the worldwide . Early and accurate detection of A . flavus is essential to reduce the damage of Aflatoxins produced by A . flavus . Based on the difference of rDNA ITS sequences among A . flavus and other Aspergillus spp .,a pair of specific primers ,S1/X2 ,was designed in this study . The primer amplified a single 334 bp product from all isolates of A . flavus and that were not from other four Aspergillus species and 17 other fungi and bacteria isolates tested . The detection sensitivity was 280fg of genomic DNA . The PCR-based detection method developed here could also be used to detect A . flavus from naturally infected peanut or corn tissues .