CAJ | 학술논문

以文心兰‘小樱桃’花梗腋芽诱导出的丛生芽为增殖培养材料，采用正交设计法，研究基本培养基、6-BA 、 NAA 和水解乳蛋白（LH）4种因素对文心兰丛生芽增殖的影响，以期筛选出各因子的最佳水平，建立文心兰‘小樱桃’丛生芽增殖培养体系。结果表明：各试验因素对文心兰丛生芽增殖影响的主次关系为6-BA ＞基本培养基＞ NAA ＞ LH ；筛选出文心兰丛生芽增殖的最佳培养基配方为改良1号＋6-BA 3.0 mg ? L -1＋ NAA 0.1 mg ? L -1＋ LH 0.5 g ? L -1，45 d 平均增殖系数达6.53，有效提高了繁殖效率，为其种苗工程化育苗提供了技术基础。
이문심란‘소앵도’화경액아유도출적총생아위증식배양재료，채용정교설계법，연구기본배양기、6-BA 、 NAA 화수해유단백（LH）4충인소대문심란총생아증식적영향，이기사선출각인자적최가수평，건립문심란‘소앵도’총생아증식배양체계。결과표명：각시험인소대문심란총생아증식영향적주차관계위6-BA ＞기본배양기＞ NAA ＞ LH ；사선출문심란총생아증식적최가배양기배방위개량1호＋6-BA 3.0 mg ? L -1＋ NAA 0.1 mg ? L -1＋ LH 0.5 g ? L -1，45 d 평균증식계수체6.53，유효제고료번식효솔，위기충묘공정화육묘제공료기술기출。
Peduncle axillary buds of the Little Cherry ,Oncidium ,were induced as explants . The orthogonal design was used to evaluate the effects of the culture medium containing inorganic substances ,6-BA ,NAA and LH on the propagation of Oncidium buds . The result showed that 6-BA ,the base medium ,NAA and LH affected significantly on the bud multiplication . The optimal medium was the Improved Formulation No .1 ,which consisted of the base medium ,6-BA 3.0 mg ? L - 1 ,NAA 0.1 mg ? L - 1 and LH0.5 g ? L - 1 ,and the average propagation coefficient was 6.53 in 45 days . The breeding efficiency was effectively improved . The result appeared to provide a basis for the commercialization of the seedling propagation .