CAJ | 학술논문

目的：比较两个封闭群SPF级昆明（ KM）小鼠的遗传差异，调查引进的SPF级KM小鼠封闭繁殖6年后，其遗传构成是否发生变化。方法应用微卫星DNA标记方法对18个位点在两个群体中的遗传差异进行分析，主要包括观察等位基因数（ Na）、有效等位基因数（ Ne）、观察杂合度（ Ho）、期望杂合度（ He）、多态信息含量（PIC ）、Shan-non信息指数、遗传分化系数（ F st）、遗传距离等遗传参数。结果两个封闭群SPF 级KM小鼠在18个微卫星位点共发现67个等位基因，Na为2～8个，平均3．7222个；Ne为1．9459～6．5442，平均2．7966个；Ho为0．4225～1．0000，平均0．8823；He为0．4892～0．8527，平均0．6162；Shannon信息指数0．6792～1．9526，平均1．0598；PIC为0．3680～0．8301，平均0．5317；Fst平均值为0．0159，表明群体间的遗传差异仅1．59％，二者间的遗传距离（DA）为0．0499。结论两个封闭群SPF级KM小鼠遗传结构相似度极高，它们与原引进群体的分化差异极小。
목적：비교량개봉폐군SPF급곤명（ KM）소서적유전차이，조사인진적SPF급KM소서봉폐번식6년후，기유전구성시부발생변화。방법응용미위성DNA표기방법대18개위점재량개군체중적유전차이진행분석，주요포괄관찰등위기인수（ Na）、유효등위기인수（ Ne）、관찰잡합도（ Ho）、기망잡합도（ He）、다태신식함량（PIC ）、Shan-non신식지수、유전분화계수（ F st）、유전거리등유전삼수。결과량개봉폐군SPF 급KM소서재18개미위성위점공발현67개등위기인，Na위2～8개，평균3．7222개；Ne위1．9459～6．5442，평균2．7966개；Ho위0．4225～1．0000，평균0．8823；He위0．4892～0．8527，평균0．6162；Shannon신식지수0．6792～1．9526，평균1．0598；PIC위0．3680～0．8301，평균0．5317；Fst평균치위0．0159，표명군체간적유전차이부1．59％，이자간적유전거리（DA）위0．0499。결론량개봉폐군SPF급KM소서유전결구상사도겁고，타문여원인진군체적분화차이겁소。
Objective Compare genetic differences between the SPF KM mice in two closed colonies ( group) and investi-gate whether the SPF KM mice occur significant changes in gene structure after their ’ s reproduction in a certain long time . Method Analysis of the genetic differences of 18 locus in the two groups was carried out by the method of labelling micro-satellite DNA markers, which includes observation of number of alleles (Na), effective number of alleles (Ne), observa-tion heterozygosity (Ho), expected heterozygosity (He), polymorphic information content (PIC), Shannon's information index, coefficient of genetic differentiation , genetic distance and so on .Result We found 67 allelles in 18 microsatellite loci of SPF KM mice in two closed colonies , Na with 2-8 and mean 3.722 2 at each locus ;Ne is 1.945 9-6.544 2, with an average of 2.796 6;Ho is 0.422 5-1.000 0, with an average of 0.882 3;He is 0.489 2-0.852 7, with an average of 0.616 2;Shannon's information index is 0.679 2-1.952 6, average 1.059 8;PIC is 0.368 0-0.830 1, with an average of 0.531 7;the average of FST is 0.015 9, indicating that only 1.59% genetic difference from SPF KM mice in two closed colonies, (a difference of 98.41%from within populations;) genetic distance (DA) between the two groups is 0.049 9. Conclusion The genetic structure of SPF KM mice is in a very high homogenecity in two closed colonies and the veriation is extremely tiny in two closed colonies and the original population .