CAJ | 학술논문

目的：利用抗心肌型脂肪酸结合蛋白单抗，研制定量检测心肌型脂肪酸结合蛋白（ H-FABP ）的ELISA试剂盒。方法使用基因重组H-FABP免疫小鼠，以杂交瘤技术制备特异性抗H-FABP单抗，用这些单抗研制定量检测H-FABP的ELISA 试剂盒。结果筛选获得2株稳定分泌抗H-FABP单抗的杂交瘤细胞株，研制了定量检测H-FABP的ELISA试剂盒，灵敏度达到0．2 ng/mL，线性范围0．4～25 ng/mL，r2＝0．9967，回收率在97．2％～104．5％，精密度的变异系数（CV）≤6．72％；应用此试剂盒检测健康人血浆H-FABP，含量为1．87～8．50 ng/mL。结论所研制的ELISA试剂盒有较好的灵敏度及特异性，可用于人血浆中H-FABP含量的检测。
목적：이용항심기형지방산결합단백단항，연제정량검측심기형지방산결합단백（ H-FABP ）적ELISA시제합。방법사용기인중조H-FABP면역소서，이잡교류기술제비특이성항H-FABP단항，용저사단항연제정량검측H-FABP적ELISA 시제합。결과사선획득2주은정분비항H-FABP단항적잡교류세포주，연제료정량검측H-FABP적ELISA시제합，령민도체도0．2 ng/mL，선성범위0．4～25 ng/mL，r2＝0．9967，회수솔재97．2％～104．5％，정밀도적변이계수（CV）≤6．72％；응용차시제합검측건강인혈장H-FABP，함량위1．87～8．50 ng/mL。결론소연제적ELISA시제합유교호적령민도급특이성，가용우인혈장중H-FABP함량적검측。
Objective To prepare monoclonal antibody against human heart-type fatty acid binding protein ( H-FABP ) and to establish a quantitative ELISA Kit in detection of H-FABP .Methods It was by immunization of mice with recombinant H-FABP ,and cell fusion was processed to establish hybridoma cell lines , the specific monoclonal antibodies against H-FABP were prepared .Results The quantitative ELISA in detection of H-FABP was developed by using these mono-clonal antibodies.The sensitivity of this assay reaches to 0.2 ng/mL, and the linear range is in 0.5-32 ng/mL with r2 val-ue of 0.996 7 and recovery rates are in 97.2%-104.5%,the CV of precision is less than 6.72%.It can measure H-FABP level in healthy human plasma in concentration 1.87-8.50 ng /mL.Conclusion The developed ELISA kit has better sensitivity and specificity , which could be used in detection of H-FABP in healthy human plasma .