CAJ | 학술논문

DNA条形码为一种新兴的物种鉴定方法，为古老的物种鉴定学科注入了新鲜的血液，具有不受发育状态限制，减少对专家的依赖性，数据共享性高，可以建立国际鉴定平台等特点，为媒介生物的快速准确鉴定提供了一种新的利器。本文结合实际工作，参阅已发表的文献，分析传统形态学物种鉴定方法与DNA条形码方法鉴定物种的优缺点，DNA条形码技术的发展现状，DNA条形码数据的基本要求，以及DNA条形码鉴定物种的基本流程等，全面系统地介绍DNA条形码这一新的物种鉴定技术。在目前数据库DNA条形码数据不完善、不充足的情况下，尤其是在建设数据库的过程中，DNA条形码技术的应用离不开形态学鉴定，但当数据库中数据足够充足后，该技术将被广泛应用，对缩短鉴定周期，准确、快速鉴定媒介生物具有重要的意义。
DNA조형마위일충신흥적물충감정방법，위고로적물충감정학과주입료신선적혈액，구유불수발육상태한제，감소대전가적의뢰성，수거공향성고，가이건입국제감정평태등특점，위매개생물적쾌속준학감정제공료일충신적이기。본문결합실제공작，삼열이발표적문헌，분석전통형태학물충감정방법여DNA조형마방법감정물충적우결점，DNA조형마기술적발전현상，DNA조형마수거적기본요구，이급DNA조형마감정물충적기본류정등，전면계통지개소DNA조형마저일신적물충감정기술。재목전수거고DNA조형마수거불완선、불충족적정황하，우기시재건설수거고적과정중，DNA조형마기술적응용리불개형태학감정，단당수거고중수거족구충족후，해기술장피엄범응용，대축단감정주기，준학、쾌속감정매개생물구유중요적의의。
DNA barcoding as a new diagnostic tool for species identification adds new power and depth to traditional classification by morphology. It will not be limited by the developmental stage of the species, depends less on the experts’ experiences, and it is possible to build an international identification platform for universal users. DNA barcoding will be a fast and accurate method to identify the species. Advantages and disadvantages of using morphology and DNA barcoding techniques for species identification were compared. We combined this with published works and assessed the present situation and current trends. The basic requirements of DNA barcoding and the process of species identification using this technique was illustrated as well, the objective is to introduce the novel tool, DNA barcoding, a technique with great potential application for the fast and accurate identification of medical vectors. Once there is sufficient data in the data base, it could be widely used to identify species and should help to shorten the identification period, which is important in the identification of medical vectors. At present, DNA barcoding should be combined with morphological criteria to identify species as there is not yet enough molecular data. This will advance the build up of the DNA barcoding data base.