CAJ | 학술논문

对《保健食品检验与评价技术规范》(2003年版)中保健食品中原花青素的测定方法进行了改进,建立了快速检测保健品中原花青素的方法。保健品中的原花青素用甲醇提取后，将试样溶液和正丁醇：盐酸（95：5,v/v）以及硫酸铁铵溶液混合置于20mL顶空瓶中，100℃烘箱中反应40min后，在546nm比色测定原花青素的含量。结果表明：原花青素在0-200μg/mL内线性良好，相关系数（r）为0.997。在1、2、5 g/kg 3个添加水平下进行了回收率和精密度试验,加标回收率为83.5%-97.5%,相对标准偏差（RSD，n=10)为3.12%-4.67%，满足检测标准要求。同时比较了两种方法的检测结果，发现没有明显差异，但改进后方法操作简便、快速，可用于保健品中原花青素的测定，能满足日常检测要求。
대《보건식품검험여평개기술규범》(2003년판)중보건식품중원화청소적측정방법진행료개진,건립료쾌속검측보건품중원화청소적방법。보건품중적원화청소용갑순제취후，장시양용액화정정순：염산（95：5,v/v）이급류산철안용액혼합치우20mL정공병중，100℃홍상중반응40min후，재546nm비색측정원화청소적함량。결과표명：원화청소재0-200μg/mL내선성량호，상관계수（r）위0.997。재1、2、5 g/kg 3개첨가수평하진행료회수솔화정밀도시험,가표회수솔위83.5%-97.5%,상대표준편차（RSD，n=10)위3.12%-4.67%，만족검측표준요구。동시비교료량충방법적검측결과，발현몰유명현차이，단개진후방법조작간편、쾌속，가용우보건품중원화청소적측정，능만족일상검측요구。
A method of the criterion of testing and evaluating health foods (2003 Edition) for the determination of procyanidin in health foods was improved. The procyanidins of health foods were extracted with methanol, the sample solutions and n-butanol:hydrochloric acid(95:5, v/v)and ammonium ferric sulfate were mixed in 20 mL headspace vial, in heat chambers at 100℃for 40 min, procyanidins were determinated by colorimetry at the 546 nm respectively. The results showed that there was a good linearity between 0-200μg/mL with the correlation coefficient (r) of 0.997, and the recovery was 83.5%-97.5%with the relative standard deviation (RSD) (n=10) of 3.12%-4.67%at the three spiked levels of 1.0, 2.0, 5.0 g/kg. At the same time, compared with the results of the two methods, and there was insignificant difference. The improved method was simple, rapid, and applicable in the determination of procyanidin in dairy products and can meets the requirements of the daily testing.