现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2013年
15期
9-10,12
,共3页
童婷%阎晓菲%张新玲%窦秉德
童婷%閻曉菲%張新玲%竇秉德
동정%염효비%장신령%두병덕
小麦%雌性育性%穗粒数%关联分析%双向极端群体
小麥%雌性育性%穗粒數%關聯分析%雙嚮極耑群體
소맥%자성육성%수립수%관련분석%쌍향겁단군체
wheat%female fertility%kernel number per spike%association mapping%diverse extreme population
为了研究小麦穗粒数与小麦雌性育性之间的关系,以含小麦雌性不育系(S)的由小麦品种(系)组成的含S种质双向极端小群体和不含小麦雌性不育系的品种双向极端小群体各60个品种(系)为目标群体,以小麦基因组中42个多态性SSR 为背景控制标记,用STRUCTRE软件进行群体结构评估,并利用TASSEL 软件的MLM模型检测210个多态性标记与小麦雌性育性I.F.(国际法育性)和D.F.(国内法育性)2种表型值的关联程度,确定入选标记,再对入选标记所在的连锁群进行分析,找到与小麦穗粒数(小穗基部小花数)更加紧密关联的标记。经关联分析,在2个极端小群体里发现,小麦小穗基部小花数关联的标记共有2个(Xgwm570-6A ,Xwmc776-4A );2个品种群体中与小麦穗粒数关联的标记共有2个(Xwmc25-2B/2D,Xwmc168-7A)。认为2D、5B连锁群可能有小麦穗粒数形成的遗传位点。
為瞭研究小麥穗粒數與小麥雌性育性之間的關繫,以含小麥雌性不育繫(S)的由小麥品種(繫)組成的含S種質雙嚮極耑小群體和不含小麥雌性不育繫的品種雙嚮極耑小群體各60箇品種(繫)為目標群體,以小麥基因組中42箇多態性SSR 為揹景控製標記,用STRUCTRE軟件進行群體結構評估,併利用TASSEL 軟件的MLM模型檢測210箇多態性標記與小麥雌性育性I.F.(國際法育性)和D.F.(國內法育性)2種錶型值的關聯程度,確定入選標記,再對入選標記所在的連鎖群進行分析,找到與小麥穗粒數(小穗基部小花數)更加緊密關聯的標記。經關聯分析,在2箇極耑小群體裏髮現,小麥小穗基部小花數關聯的標記共有2箇(Xgwm570-6A ,Xwmc776-4A );2箇品種群體中與小麥穗粒數關聯的標記共有2箇(Xwmc25-2B/2D,Xwmc168-7A)。認為2D、5B連鎖群可能有小麥穗粒數形成的遺傳位點。
위료연구소맥수립수여소맥자성육성지간적관계,이함소맥자성불육계(S)적유소맥품충(계)조성적함S충질쌍향겁단소군체화불함소맥자성불육계적품충쌍향겁단소군체각60개품충(계)위목표군체,이소맥기인조중42개다태성SSR 위배경공제표기,용STRUCTRE연건진행군체결구평고,병이용TASSEL 연건적MLM모형검측210개다태성표기여소맥자성육성I.F.(국제법육성)화D.F.(국내법육성)2충표형치적관련정도,학정입선표기,재대입선표기소재적련쇄군진행분석,조도여소맥수립수(소수기부소화수)경가긴밀관련적표기。경관련분석,재2개겁단소군체리발현,소맥소수기부소화수관련적표기공유2개(Xgwm570-6A ,Xwmc776-4A );2개품충군체중여소맥수립수관련적표기공유2개(Xwmc25-2B/2D,Xwmc168-7A)。인위2D、5B련쇄군가능유소맥수립수형성적유전위점。
To study the relationship of female fertility and kernel number per spike in wheat ,two small populations with 60 varieties or lines alternatively ,one called S involved (female sterile line S )diverse extreme population and another called variety diverse extreme population ,were composed as tested populations ,42 polymorphic SSR markers in wheat genome were selected as background marker stotest. Its′ population genetic structure,the MLM mode lins of tware TASSEL was used to check 210 polymorphic markers association degree with phenotype female fertility (international fertility-I.F. and domestic fertility-D.F ),the highly associated markers with phenotype from small population analysis were verified in relatively big variety population and S involved bigger population ,find highly associated markers with kernel number per spike in wheat. Total 2 (Xgwm570-6A,Xwmc776-4A)association markers were found by two small extreme populations with phenotype kernel number per spike. Total 2 (Xwmc25-2B/2D,Xwmc168-7A)association markers were found by two small variety populations with phenotype kernel number per spike. It was believed that 2D and 5B loci were associatied with kernel number per spike.