CAJ | 학술논문

采用高效液相色谱法建立了同时测定何首乌中大黄酸、大黄素、大黄酚三种蒽醌类化合物的方法,样品采用超声波法提取,色谱柱为Phenomenex C18(250 mm×4.6 mm,5μm)；流动相为甲醇-0.4%醋酸水溶液(80∶20)；流速0.80 mL/min；紫外检测波长254 nm；柱温为35℃.结果：该方法对大黄酸、大黄素和大黄酚分别在0.0001~12.50μg/mL、0.0002~25.00μg/mL、0.00018~22.50μg/mL 范围内线性关系良好,相关系数(r)均大于0.9999,定性检测限(S/N=3)依次是：0.0703 ng/mL、0.0282 ng/mL、0.0871 ng/mL；样品回收率为88.39%~107.2%.结论：本法操作简单快速、定量准确、灵敏度高、成本低,为何首乌中蒽醌类化合物的检测分离及含量测定提供了一个有效的科学方法.
채용고효액상색보법건립료동시측정하수오중대황산、대황소、대황분삼충은곤류화합물적방법,양품채용초성파법제취,색보주위Phenomenex C18(250 mm×4.6 mm,5μm)；류동상위갑순-0.4%작산수용액(80∶20)；류속0.80 mL/min；자외검측파장254 nm；주온위35℃.결과：해방법대대황산、대황소화대황분분별재0.0001~12.50μg/mL、0.0002~25.00μg/mL、0.00018~22.50μg/mL 범위내선성관계량호,상관계수(r)균대우0.9999,정성검측한(S/N=3)의차시：0.0703 ng/mL、0.0282 ng/mL、0.0871 ng/mL；양품회수솔위88.39%~107.2%.결론：본법조작간단쾌속、정량준학、령민도고、성본저,위하수오중은곤류화합물적검측분리급함량측정제공료일개유효적과학방법.
A sensitive and specific high performance liquid chromatography (HPLC)method is developed to determine three anthraquinones,Rhein,Emodin and Chrysophanol,in Polygonum Multiflorum.The three compounds are obtained by ultrasonic extraction,separated on Phenomenex C18 (250 mm×4.6 mm,5μm)with a mixture of methanol -0.4% HAC (volume ration 80:20)as mobile phase at flow rate 0.8 mL/min.The UV detective wavelength is 254 nm,and the column temperature is set at 35 ℃. Result:The calibration curves for Rhein,Emodin and Chrysophanol are linear between 0.0001~12.50μg/mL (r=0.99996),0.0002~25.00 μg/mL (r=0.99999)and 0.00018~22.50 μg/mL (r=0. 99993),respectively.The limits of detection (LOD)are 0.073 ng/mL,0.0282 ng/mL and 0.0871 ng/mL,respectively.The recoveries of the three compounds range are between 88.39%~107.2%.The re-sults indicate that the proposed method is simple,rapid,accurate,sensitive,and low cost,thus it pro-vides an effective scientific means for assaying anthraquinones in Polygonum Multifloru.