CAJ | 학술논문

为了研究对氯酚（4-CP）、2，4-二氯酚（2，4-DCP）、2，4，6-三氯酚（2，4，6-TCP）对斑马鱼的生理毒性作用，以7 d富集系数（ KBCF ）和超氧化物歧化酶（ SOD）活性为评价指标，采用半静态试验法对斑马鱼进行驯养和试验，测定斑马鱼对3种氯酚的7 d KBCF ，同时测定不同浓度条件下，暴露1 d和7 d，斑马鱼体内SOD酶活性。结果表明，4-CP、2，4-DCP和2，4，6-TCP的7 d KBCF分别为33.65±5.29、65.54±10.49和128.05±39.83，富集能力表现为4-CP （无富集）＜2，4-DCP（轻度富集）＜2，4，6-TCP（轻度富集）；斑马鱼对氯代酚类物质的富集性与氯酚类物质氯原子取代个数正相关。3种氯酚胁迫斑马鱼结果表明，暴露1d表现为诱导-抑制效应，暴露7d表现为抑制效应，酶活性变化显著。
위료연구대록분（4-CP）、2，4-이록분（2，4-DCP）、2，4，6-삼록분（2，4，6-TCP）대반마어적생리독성작용，이7 d부집계수（ KBCF ）화초양화물기화매（ SOD）활성위평개지표，채용반정태시험법대반마어진행순양화시험，측정반마어대3충록분적7 d KBCF ，동시측정불동농도조건하，폭로1 d화7 d，반마어체내SOD매활성。결과표명，4-CP、2，4-DCP화2，4，6-TCP적7 d KBCF분별위33.65±5.29、65.54±10.49화128.05±39.83，부집능력표현위4-CP （무부집）＜2，4-DCP（경도부집）＜2，4，6-TCP（경도부집）；반마어대록대분류물질적부집성여록분류물질록원자취대개수정상관。3충록분협박반마어결과표명，폭로1d표현위유도-억제효응，폭로7d표현위억제효응，매활성변화현저。
To study the physio-toxicity effect of 4-chlorophenol, 2,4-dichlorophenol and 2,4,6-trichlorphenol on zebrafish, the evaluation indicator of 7 d KBCF and SOD activity with different exposure concentrations were determined using zebrafish by semi-static test.The results showed that the 7 d KBCF of 4-CP, 2, 4-DCP and 2,4,6-TCP to zebrafish were 33.65 ±5.29, 65.54 ±10.49 and 128.05 ±39.83, respectively, and the bioconcentration factor followed the order of 4-CP <2, 4-DCP <2, 4, 6-TCP. The bioconcentration of the chlorophenols to zebrafish and the numbers of chloro derivatives had positive correlation .The results of stress action test of the chlorophenols to zebrafish showed that the effect at 1 d was induction-inhibition and at 7 d the effect was inhibition , indicating significant variation of SOD activity .