CAJ | 학술논문

目的：观察非酒精性脂肪性肝病（NAFLD）大鼠血清胰岛素样生长因子1（IGF-1）、胰岛素样生长因子结合蛋白3（ IGFBP-3）的表达及多烯磷脂酰胆碱对其的影响。方法采用高脂饲料喂养建立大鼠NAFLD模型，以多烯磷脂酰胆碱进行干预。 HE染色动态观察大鼠4、8、12周时肝组织病理学变化，IRMA法动态监测血清IGF-1、IGFBP-3含量。结果正常组大鼠各时间点肝组织病理均无明显异常；模型组随高脂饮食时间延长，在4、8、12周3个时相点肝组织脂肪变、炎症程度、气球样变及NAFLD活动度积分逐渐增强，血清IGF-1、IGFBP-3逐渐明显下降，且模型组各时相点IGF-1、IGFBP-3的水平较正常组同一时相点明显降低；应用多烯磷脂酰胆碱干预后，大鼠肝组织炎症程度、NAFLD活动度积分较模型组显著降低，IGF-1、IGFBP-3的水平则较模型组明显增高。结论血清IGF-1、IGFBP-3水平随大鼠NAFLD进展而降低。
목적：관찰비주정성지방성간병（NAFLD）대서혈청이도소양생장인자1（IGF-1）、이도소양생장인자결합단백3（ IGFBP-3）적표체급다희린지선담감대기적영향。방법채용고지사료위양건립대서NAFLD모형，이다희린지선담감진행간예。 HE염색동태관찰대서4、8、12주시간조직병이학변화，IRMA법동태감측혈청IGF-1、IGFBP-3함량。결과정상조대서각시간점간조직병리균무명현이상；모형조수고지음식시간연장，재4、8、12주3개시상점간조직지방변、염증정도、기구양변급NAFLD활동도적분축점증강，혈청IGF-1、IGFBP-3축점명현하강，차모형조각시상점IGF-1、IGFBP-3적수평교정상조동일시상점명현강저；응용다희린지선담감간예후，대서간조직염증정도、NAFLD활동도적분교모형조현저강저，IGF-1、IGFBP-3적수평칙교모형조명현증고。결론혈청IGF-1、IGFBP-3수평수대서NAFLD진전이강저。
Objective To observe the expression of insulin-like growth factor 1 (IGF-1),insulin-like growth factor binding protein 3 ( IGFBP-3) in rats’ serum with non-alcoholic fatty liver disease ( NAFLD) and the impact of Polyene phosphatidyl choline .Methods NAFLD model was induced by feeding the SD rats with a high-fat diet, with Polyene phosphatidyl choline to intervene .Observe the pathological changes of the rats ’ liver tissue dynamically after HE staining . Detect the contents of IGF-1,IGFBP-3 in serum dynamically through the immuno radio metric assay ( IRMA).Results There were no obvious exception of the liver tissue pathology in the normal group at each time .With the extension of a high-fat diet,the liver tissue of the model group increased on fatty change ,the degree of inflammation ,the balloon sample change , the NAFLD activity score at 4,8,12weeks.IGF-1,IGFBP-3 in serum were decreased significantly ,and the model set was significantly lower than the normal group at the same phase .After the application of polyene phosphatidyl choline ,the degree of rat liver tissue inflammation and the NAFLD activity score were reduced significantly when compared with model group , while the level of IGF-1,IGFBP-3were significantly higher .Conclusion The levels of IGF-1,IGFBP-3 in the rats’ serum reduce in the development of non-alcoholic fatty liver disease .