CAJ | 학술논문

目的：对Leptin基因敲除SD大鼠血糖变化及病理表型进行长期分析，为使用该大鼠作为糖尿病和脂代谢模型积累数据。方法 western blot检测Leptin＋/＋大鼠和Leptin-/-大鼠肝脏中Leptin的表达。利用称量方法测定Leptin基因敲除的SD大鼠（Leptin-/-）1，3，6，8月龄的体重变化，利用稳豪血糖仪采尾血测定1，3，6，8月龄Leptin-/-大鼠空腹血糖值。 HE染色和免疫组织化学观察Leptin-/-大鼠胰腺及肝脏的病理学变化。结果 Leptin-/-大鼠肝脏中表达变短的功能异常的Leptin蛋白。 Leptin-/-大鼠从1月龄开始出现显著的体重增加，8月龄时雌性体重为884 g，雄性体重可以达到1200 g，是野生SD大鼠的2倍。自1月龄起， Leptin-/-雌鼠的空腹血糖值显著高于野生大鼠，在1月龄到6月龄之间差别明显（40％～26％），到8月龄和野生大鼠恢复到近正常水平。8月龄Leptin-/-大鼠肝脏肝小叶中出现大量脂肪空泡，胰腺内较多脂肪细胞浸润、胰岛数量明显增多，体积增大，胰岛素阳性β细胞增多。结论 Leptin-/-大鼠表型表现为肥胖，脂肪肝，胰岛增生和早期高血糖。
목적：대Leptin기인고제SD대서혈당변화급병리표형진행장기분석，위사용해대서작위당뇨병화지대사모형적루수거。방법 western blot검측Leptin＋/＋대서화Leptin-/-대서간장중Leptin적표체。이용칭량방법측정Leptin기인고제적SD대서（Leptin-/-）1，3，6，8월령적체중변화，이용은호혈당의채미혈측정1，3，6，8월령Leptin-/-대서공복혈당치。 HE염색화면역조직화학관찰Leptin-/-대서이선급간장적병이학변화。결과 Leptin-/-대서간장중표체변단적공능이상적Leptin단백。 Leptin-/-대서종1월령개시출현현저적체중증가，8월령시자성체중위884 g，웅성체중가이체도1200 g，시야생SD대서적2배。자1월령기， Leptin-/-자서적공복혈당치현저고우야생대서，재1월령도6월령지간차별명현（40％～26％），도8월령화야생대서회복도근정상수평。8월령Leptin-/-대서간장간소협중출현대량지방공포，이선내교다지방세포침윤、이도수량명현증다，체적증대，이도소양성β세포증다。결론 Leptin-/-대서표형표현위비반，지방간，이도증생화조기고혈당。
Objective To obtain more physiological data of Leptin knockout SD rats available for the user , the long-term observation of fasting blood glucose and pathological phenotypes were performed .Methods The protein expression levels in liver tissues were determined by western blot .Body weight of Leptin knockout rats ( Leptin-/-) and littermate lean rats (Leptin+/+) were weighed up at 1,3,6,8 months of age.Fasting blood glucose of Leptin +/+rats and Leptin-/-rats at 1,3,6,8 months of age were measured using One Touch? brand blood glucose monitoring systems.Pathological changes of pancreas and livers of Leptin -/-rats were observed by the method of HE staining and Immunohistochemistry (IHC).Results Short null Lepin proteins were expressed in liver tissues from Leptin -/-rats. Leptin-/-rats become heavier than Leptin +/+rats since they were one month old .The body weight of Leptin -/-rats at 8 months of age was twice as heavy as Leptin +/+rats, female Leptin-/-rats weighing 884g, and male Leptin-/-rats weighing 1200g.Overt hyperglycemia was observed during the first month after birth .Compared with Leptin+/+female rats,the fasting blood glucose of Leptin -/-female rats was increased by 40%-26%from 1 to 6 months old. After that, blood glucose values decreased and eventually become nearly normal at 8 months of age.Pathological examination indicated that Leptin -/-rats at 8 months of age had a fatty liver , more pancreas islets with lager volume and more beta cells with increased insulin secretion .Conclusion Leptin-/-rat were characterized by obesity , fatty liver, islet cell hyperplasia and early hyperglycemia .