CAJ | 학술논문

目的：利用IL-33转基因小鼠研究IL-33对造血干/祖细胞的增殖和分化影响。方法利用流式细胞仪分析IL-33转基因小鼠及同窝野生对照小鼠的外周血、脾脏、骨髓细胞的免疫表型及造血干细胞分化不同阶段细胞的数量变化；利用体外成克隆实验和细胞周期分析研究IL-33对于造血干细胞增殖能力的影响。结果与野生型小鼠相比，IL-33转基因小鼠B细胞和T细胞在外周血中都明显降低，粒细胞在外周血和骨髓中都有明显增加；IL-33转基因小鼠的骨髓造血干细胞和多能祖细胞数量减少，共同淋系祖细胞数量减少，共同髓系祖细胞和粒单系祖细胞数量增加；IL-33转基因小鼠的造血干细胞处于S-G2-M的细胞增多；体外单克隆实验发现IL-33转基因小鼠造血干细胞形成的集落数增加。结论 IL-33转基因小鼠造血干细胞增殖能力增强，更易向髓系细胞分化。
목적：이용IL-33전기인소서연구IL-33대조혈간/조세포적증식화분화영향。방법이용류식세포의분석IL-33전기인소서급동와야생대조소서적외주혈、비장、골수세포적면역표형급조혈간세포분화불동계단세포적수량변화；이용체외성극륭실험화세포주기분석연구IL-33대우조혈간세포증식능력적영향。결과여야생형소서상비，IL-33전기인소서B세포화T세포재외주혈중도명현강저，립세포재외주혈화골수중도유명현증가；IL-33전기인소서적골수조혈간세포화다능조세포수량감소，공동림계조세포수량감소，공동수계조세포화립단계조세포수량증가；IL-33전기인소서적조혈간세포처우S-G2-M적세포증다；체외단극륭실험발현IL-33전기인소서조혈간세포형성적집락수증가。결론 IL-33전기인소서조혈간세포증식능력증강，경역향수계세포분화。
Objective To study the influence of IL-33 on the Hematopoietic stem cells and progenitor cells . Methods Cells from the peripheral blood , spleen, thymus and bone marrow were stained with indicated antibodies and analyzed by flow cytometry . The LT-HSCs were sorted and culture using in vitro clonogenic assay . Results The percentage of B cells and T cells was decreased and the percentage of M cells was increased in the peripheral blood from IL -33 transgenic mice .Compared with the wildtype mice , the number of HSCs , MPPs and CLP was decreased;meanwhile the number of CMP and GMP was increased in the bone marrow from IL-33 transgenic mice .An in vitro clonogenic assay showed that LT-HSCs increased the ability to self-renew from IL-33 transgenic mice .And the percentage of S-G2-M stage hematopoietic stem cell was increased from IL-33 transgenic mice .Conclusion IL-33 increase the myeloid differentiation in hematopoietic stem cells .