中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
8期
1117-1120
,共4页
罗盈%王春苗%林婷%罗燕飞%甘慧泉%周茂华
囉盈%王春苗%林婷%囉燕飛%甘慧泉%週茂華
라영%왕춘묘%림정%라연비%감혜천%주무화
CD45RA%CD45RO%慢性移植物抗宿主病%流式细胞术%异基因造血干细胞移植
CD45RA%CD45RO%慢性移植物抗宿主病%流式細胞術%異基因造血榦細胞移植
CD45RA%CD45RO%만성이식물항숙주병%류식세포술%이기인조혈간세포이식
CD45RA%CD45RO%cGVHD%FCM%allo-HSCT
目的：探讨同种异基因造血干细胞移植（ allo-HSCT ）后发生慢性移植物抗宿主病（ cGVHD ）患者外周血中CD45 RA＋和CD45 RO＋T淋巴细胞亚群特点及其与cGVHD发生之间的关系。方法：回顾性研究广东省人民医院2001年3月～2009年9月64例造血干细胞移植植入成功的患者，其中无cGVHD发生21例，轻度cGVHD 15例，中度cGVHD 18例，重度cGVHD 10例，应用流式细胞术，对其外周血中CD4＋CD45RA＋、CD4＋CD45RO＋、CD8＋CD45RA＋和CD8＋CD45RO＋T淋巴细胞亚群进行检测。结果：与无cGVHD患者相比，cGVHD患者CD4＋CD45RA＋T细胞百分比均明显降低（P＜0.05），CD4＋CD45RO＋T细胞百分比均明显增高（ P＜0.05），而CD8＋CD45 RA＋、CD8＋CD45 RO＋T细胞百分比均无明显改变。轻、中、重度的cGVHD患者之间比较，CD4＋CD45RA＋、CD4＋CD45RO＋、CD8＋CD45RA＋和CD8＋CD45RO＋T细胞百分比均无明显改变。结论：CD4＋CD45RA＋和CD4＋CD45 RO＋T细胞可能对cGVHD的发生有重要意义。
目的：探討同種異基因造血榦細胞移植（ allo-HSCT ）後髮生慢性移植物抗宿主病（ cGVHD ）患者外週血中CD45 RA＋和CD45 RO＋T淋巴細胞亞群特點及其與cGVHD髮生之間的關繫。方法：迴顧性研究廣東省人民醫院2001年3月～2009年9月64例造血榦細胞移植植入成功的患者，其中無cGVHD髮生21例，輕度cGVHD 15例，中度cGVHD 18例，重度cGVHD 10例，應用流式細胞術，對其外週血中CD4＋CD45RA＋、CD4＋CD45RO＋、CD8＋CD45RA＋和CD8＋CD45RO＋T淋巴細胞亞群進行檢測。結果：與無cGVHD患者相比，cGVHD患者CD4＋CD45RA＋T細胞百分比均明顯降低（P＜0.05），CD4＋CD45RO＋T細胞百分比均明顯增高（ P＜0.05），而CD8＋CD45 RA＋、CD8＋CD45 RO＋T細胞百分比均無明顯改變。輕、中、重度的cGVHD患者之間比較，CD4＋CD45RA＋、CD4＋CD45RO＋、CD8＋CD45RA＋和CD8＋CD45RO＋T細胞百分比均無明顯改變。結論：CD4＋CD45RA＋和CD4＋CD45 RO＋T細胞可能對cGVHD的髮生有重要意義。
목적：탐토동충이기인조혈간세포이식（ allo-HSCT ）후발생만성이식물항숙주병（ cGVHD ）환자외주혈중CD45 RA＋화CD45 RO＋T림파세포아군특점급기여cGVHD발생지간적관계。방법：회고성연구광동성인민의원2001년3월～2009년9월64례조혈간세포이식식입성공적환자，기중무cGVHD발생21례，경도cGVHD 15례，중도cGVHD 18례，중도cGVHD 10례，응용류식세포술，대기외주혈중CD4＋CD45RA＋、CD4＋CD45RO＋、CD8＋CD45RA＋화CD8＋CD45RO＋T림파세포아군진행검측。결과：여무cGVHD환자상비，cGVHD환자CD4＋CD45RA＋T세포백분비균명현강저（P＜0.05），CD4＋CD45RO＋T세포백분비균명현증고（ P＜0.05），이CD8＋CD45 RA＋、CD8＋CD45 RO＋T세포백분비균무명현개변。경、중、중도적cGVHD환자지간비교，CD4＋CD45RA＋、CD4＋CD45RO＋、CD8＋CD45RA＋화CD8＋CD45RO＋T세포백분비균무명현개변。결론：CD4＋CD45RA＋화CD4＋CD45 RO＋T세포가능대cGVHD적발생유중요의의。
To characterize the CD45RA+and CD45RO+T lymphocyte subsets in the peripheral blood of patients with cGVHD induced by allogeneic haematopoietic stem cell transplantation ( allo-HSCT ) and to explore their relations with the disease.Methods:The peripheral blood was collected from 64 patients after allo-HSCT,including 21 non-cGVHD patients,15 light grade cGVHD patients,18 mild grade cGVHD patients and 10 severe grade cGVHD patients,then CD4+CD45RA+,CD4+CD45RO+, CD8+CD45RA+and CD8+CD45RO+T lymphocyte subsets were detected by flow cytometry ( FCM).Results: Compared with the control,the percent of CD4+CD45RA+T lymphocyte in patients with light,mild and severe grade cGVHD decreased markedly (P<0.05),the percent of CD4+CD45RO+T lymphocyte increased markedly (P<0.05).But there were not obviously change in the patient with different grade cGVHD.The percent of CD8+CD45RA+,CD8+CD45RO+T lymphocyte did not change obviously.Conclusion:CD4+CD45RA+and CD4+CD45RO+may play an important role in the pathogenesis of cGVHD.
