CAJ | 학술논문

目的：比较单纯胆固醇饮食与胆固醇合并玉米油饮食用于建立兔动脉粥样硬化模型的可靠性及其所形成斑块面积的大小，为兔动脉粥样硬化模型的建立方法提供基本数据。方法：新西兰兔18只，随机分为3组（每组6只），即普通饲料组（C组）、单纯高胆固醇饲料组（H1组）和高胆固醇玉米油饲料组（H2组）。实验开始前至第12周，每周末称动物体重。分别于实验开始前、第12周末时检测血清高密度脂蛋白胆固醇（ HDL-C）、低密度脂蛋白胆固醇（ LDL-C）、总胆固醇（ TC）和甘油三酯（ TG）浓度。12周末将动物麻醉后，从主动脉根部起取胸主动脉8 cm行脂肪染色，观察是否存在动脉粥样硬化斑块，并测定斑块面积（ PA）占主动脉内膜面积（ IA）百分比（PA／IA）。结果：实验第12周末，H1组和H2组的HDL-C、LDL-C和TC水平均高于C组（P＜0．05），H2组TG高于C组（P＜0．05）；H2组HDL-C水平高于H1组（P＜0．05），但2组间LDL-C、TC及TG水平无显著差异（P＞0.05）。 C组无动脉粥样硬化斑块形成，H1组和H2组兔均有动脉粥样硬化斑块，造模成功率为100％。 H1组及H2组PA／IA分别为（49．74±18．78）％和（56．95±26．74）％，2组间无显著差异（P＞0．05），但与C组相比均有显著差异（ P＜0．05）。结论：单纯高胆固醇饮食或高胆固醇加玉米油饮食均能成功建立兔动脉粥样硬化模型，饲养12周的建模成功率均为100％，且2种方法对形成斑块的面积无明显影响。
목적：비교단순담고순음식여담고순합병옥미유음식용우건립토동맥죽양경화모형적가고성급기소형성반괴면적적대소，위토동맥죽양경화모형적건립방법제공기본수거。방법：신서란토18지，수궤분위3조（매조6지），즉보통사료조（C조）、단순고담고순사료조（H1조）화고담고순옥미유사료조（H2조）。실험개시전지제12주，매주말칭동물체중。분별우실험개시전、제12주말시검측혈청고밀도지단백담고순（ HDL-C）、저밀도지단백담고순（ LDL-C）、총담고순（ TC）화감유삼지（ TG）농도。12주말장동물마취후，종주동맥근부기취흉주동맥8 cm행지방염색，관찰시부존재동맥죽양경화반괴，병측정반괴면적（ PA）점주동맥내막면적（ IA）백분비（PA／IA）。결과：실험제12주말，H1조화H2조적HDL-C、LDL-C화TC수평균고우C조（P＜0．05），H2조TG고우C조（P＜0．05）；H2조HDL-C수평고우H1조（P＜0．05），단2조간LDL-C、TC급TG수평무현저차이（P＞0.05）。 C조무동맥죽양경화반괴형성，H1조화H2조토균유동맥죽양경화반괴，조모성공솔위100％。 H1조급H2조PA／IA분별위（49．74±18．78）％화（56．95±26．74）％，2조간무현저차이（P＞0．05），단여C조상비균유현저차이（ P＜0．05）。결론：단순고담고순음식혹고담고순가옥미유음식균능성공건립토동맥죽양경화모형，사양12주적건모성공솔균위100％，차2충방법대형성반괴적면적무명현영향。
AIM:To compare the reliability and plaque area between using high-cholesterol diet and high-cho-lesterol diet with corn oil to establish a rabbit atherosclerotic model .METHODS:Eighteen New Zealand rabbits were ran-domly divided into 3 groups (6 rabbits each):normal diet group (group C), high-cholesterol diet group (group H1) and high-cholesterol diet containing 6%corn oil group (group H2).All rabbits were fed for 12 weeks, and their body mea-sured was weighed at the end of every weeks .The serum levels of high-density lipoprotein cholesterol ( HDL-C) , low-den-sity lipoprotein cholesterol ( LDL-C) , total cholesterol ( TC) and triglyceride ( TG) were measured by automatic chemistry analyzer at 0 week and 12 weeks.At the end of 12 weeks, the thoracic aorta of 8-cm length since aortic root was isolated from the rabbit after anesthesia , and stained with Sudan IV or oil red O to verify the existence of plaque .The percentage of plaque area (PA/IA) in the intima area was further calculated by ImageJ 2x software.RESULTS:At the end of 12-week feeding, the serum levels of HDL-C, LDL-C and TC in both group H1 and group H2 were significantly higher than those in group C, and serum TG in group H2 was significantly higher than that in group C .Serum HDL-C in group H2 was signifi-cantly higher than that in group H1, but no significant difference of serum LDL-C, TC and TG between group H1 and group H2 was found .There was no plaque in the intima in group C , and plaques were observed in the intima of all rabbits in group H1 and group H2.Rabbit atherosclerotic models in both group H 1 and group H2 were established with a success rate of 100%.The values of PA/IA in group H1 [(49.74 ±18.78)%] and group H2 [(56.95 ±26.74)%] were both sig-nificantly higher than that in group C (0%), and no significant difference of PA/IA between group H1 and group H2 was observed.CONCLUSION:High-cholesterol diet with or without corn oil can establish a rabbit atherosclerotic model with a success rate of 100%after 12-week feeding , and the percentage of plaque area in the total aortic intimal area is not differ-ent in the 2 feeding methods .