CAJ | 학술논문

目的：为了解雌性山羊星状神经节是否具备接受γ-干扰素（ Interferon-γ，IFN-γ）作用的条件，即是否有IFN-γ受体（ Interferon-γreceptor，IFNGR）存在。方法：取雌性山羊星状神经节，用免疫组化SP法和PCR方法检测IFNGR在星状神经节的表达情况。结果：结果显示，在雌性山羊星状的神经元、支持细胞和神经纤维均有IFNGR免疫阳性产物分布，但主要分布于神经元细胞膜与细胞核，IFNGR在神经元胞体的相对表达量极显著高于非神经细胞结构（P＜0．01）。在山羊星状神经节扩增到IFNGR1基因的cDNA片段全长376 bp，山羊IFNGR1基因与绵羊基因同源性最高（98％），其次为牛（97％）。结论：在雌性山羊星状神经节中IFNGR主要在交感节后神经元表达与分布，具备对IFN-γ刺激做出反应的条件，提示雌性山羊星状神经节可能作为IFN-γ对心血管免疫调节途径和自主神经对心血管调节的神经途径之间相互协调的关键点。
목적：위료해자성산양성상신경절시부구비접수γ-간우소（ Interferon-γ，IFN-γ）작용적조건，즉시부유IFN-γ수체（ Interferon-γreceptor，IFNGR）존재。방법：취자성산양성상신경절，용면역조화SP법화PCR방법검측IFNGR재성상신경절적표체정황。결과：결과현시，재자성산양성상적신경원、지지세포화신경섬유균유IFNGR면역양성산물분포，단주요분포우신경원세포막여세포핵，IFNGR재신경원포체적상대표체량겁현저고우비신경세포결구（P＜0．01）。재산양성상신경절확증도IFNGR1기인적cDNA편단전장376 bp，산양IFNGR1기인여면양기인동원성최고（98％），기차위우（97％）。결론：재자성산양성상신경절중IFNGR주요재교감절후신경원표체여분포，구비대IFN-γ자격주출반응적조건，제시자성산양성상신경절가능작위IFN-γ대심혈관면역조절도경화자주신경대심혈관조절적신경도경지간상호협조적관건점。
Objective:To detect the existence of IFNGR in the stellate ganglion in female goats .Methods:The stellate ganglia were taken from female goats .The expression of IFNGR was detected by PCR method and immunohistochemical SP staining .Results:The results showed that:IFNGR immunoreactive substances were distributed in neurons , supporting cells and passing fiber , and mainly in the cytomembrane and nuclei of neurons .The relative expression of IFNGR was very significantly higher than that of non-neuronal cells (P<0.01).The sequence of IFNGR1 gene amplified by PCR method contained 376 bp, and IFNGR1 gene of goats exhibited the highest homology with sheep(98%),followed by cattle(97%).Conclusion:The results suggested that the IFNGR in the Stellate Gan-glion of female goats mainly expressed and located in sympathetic postganglionic neurons which were provided with the conditions for the role of IFN-γ, which implied that the Stellate Ganglion may act as the critical point to concert the immune regulation of IFN -γand neu-roregulation of autonomic nerve on cardiovascular system .