CAJ | 학술논문

目的探讨血管紧张素Ⅱ（AngⅡ）和血管紧张素1-7（Ang1-7）对大鼠肝星状细胞Rho-Rock信号转导通路的影响。方法采用HSC-T6细胞株，分别给予AngⅡ、Ang1-7、AngⅡ+Ang1-7、Ang1-7+A77910μmol/L处理，逆转录聚合酶链反应（RT-PCR）检测Rho-Rock通路中Rock2（Rho kinase2）、RhoAGTP、RhoGEF （Rho Guanine Nucleotide Exchange Factors, RhoGEF）的表达。结果 AngⅡ处理组Rock2、RhoAGTP、RhoGEF mRNA的表达显著增强，Ang1-7组Rock2、RhoAGTP、RhoGEF mRNA的表达显著低于阴性对照组。结论 Ang1-7可抑制AngⅡ诱导的Rho-Rock信号通路的表达。
목적탐토혈관긴장소Ⅱ（AngⅡ）화혈관긴장소1-7（Ang1-7）대대서간성상세포Rho-Rock신호전도통로적영향。방법채용HSC-T6세포주，분별급여AngⅡ、Ang1-7、AngⅡ+Ang1-7、Ang1-7+A77910μmol/L처리，역전록취합매련반응（RT-PCR）검측Rho-Rock통로중Rock2（Rho kinase2）、RhoAGTP、RhoGEF （Rho Guanine Nucleotide Exchange Factors, RhoGEF）적표체。결과 AngⅡ처리조Rock2、RhoAGTP、RhoGEF mRNA적표체현저증강，Ang1-7조Rock2、RhoAGTP、RhoGEF mRNA적표체현저저우음성대조조。결론 Ang1-7가억제AngⅡ유도적Rho-Rock신호통로적표체。
Objective To investigate the effects of Angiotensin1-7 on Rho-Rock pathway of rat hepatic stellate cell. Methods HSC-T6 cells were treated with 10μmol/L of AngII, Ang1-7,AngⅡ+Ang1-7, and Ang1-7+A779, respectively. RT-PCR was used to detect the expression of Rock2,RhoAGTP and RhoGEF in Ca2+-independent pathways mediated by Rho-kinase. Results The mRNA expression of Rock2, RhoAGTP, and RhoGEF was significantly increased after AngII treatment (P<0.01), but decreased following subsequent Ang1-7 treatment. Conclusion Ang1-7 could inhibit AngⅡ-induced activation of the Rho-Rock pathway.