CAJ | 학술논문

分别采用超声、回流、浸泡法分离提取万寿菊中的叶黄素，再将其皂化提纯后进行液相测定，选出最佳分离提取方法。采用ODS C18填料色谱柱（4.6×250mm ，5μm ），柱温30℃，流速1mL/min ，进样10μL ，时间：22min ，流动相A （乙腈∶甲醇＝9∶1）∶B （乙酸乙酯）。样品采用梯度洗脱，0～5min ，B 10％；5～15min ，B 15％；15～20min ，B 0％。检测波长450nm。由叶黄素得率可知，浸泡提取法可作为提取万寿菊颗粒中叶黄素的有效实验方法，而且浸泡16h万寿菊中的叶黄素得率最多。叶黄素的质量浓度与峰面积分别在0.1～0.6mg/mL （ r＝0.9997）呈良好线性。通过标准品和样品的液相色谱图比较，确定皂化后所得的样品为叶黄素。证明此实验为最佳叶黄素的提取皂化实验。
분별채용초성、회류、침포법분리제취만수국중적협황소，재장기조화제순후진행액상측정，선출최가분리제취방법。채용ODS C18전료색보주（4.6×250mm ，5μm ），주온30℃，류속1mL/min ，진양10μL ，시간：22min ，류동상A （을정∶갑순＝9∶1）∶B （을산을지）。양품채용제도세탈，0～5min ，B 10％；5～15min ，B 15％；15～20min ，B 0％。검측파장450nm。유협황소득솔가지，침포제취법가작위제취만수국과립중협황소적유효실험방법，이차침포16h만수국중적협황소득솔최다。협황소적질량농도여봉면적분별재0.1～0.6mg/mL （ r＝0.9997）정량호선성。통과표준품화양품적액상색보도비교，학정조화후소득적양품위협황소。증명차실험위최가협황소적제취조화실험。
Ultrasound ,reflux and soaking methods were used for separation of lutein marigold extract and then purified by liquid saponification its determination .The best method of separation and extraction was chosen.ODS C18 column using filler(4.6 × 250mm ,5μm) ,Column temper-ature at 30℃ ,flow at 1mL/min ,sample 10μL ,time:22min ,mobile phase A (acetonitrile∶methanol=9∶1 )∶B (ethyl acetate ) .Samples using a gradient elution ,0~5min when B Mobile phase at 10% ,5~15min when B Mobile phase was set at 15% ,15~20min when B Mobile phase was 0% ,the detection wavelength was at 450nm .Soaked by the lutein extraction yield can be seen as the extraction of lutein marigold particles effective experimental methods and soak 16h marigold lutein yield most .Lutein concentration and peak area ,respectively 0.1~0.6mg/mL( r=0.999 7 ) showed good linearity .Standards and samples by liquid chromatogram determined by comparing the sample obtained after saponifica-tion of lutein .This experiment proved the best lutein extraction saponification experiments .