CAJ | 학술논문

通过MVA与MEP途径的抑制剂洛伐他汀和膦胺霉素不同浓度及作用时间对人参发根生长影响的研究，确定洛伐他汀和膦胺霉素对人参总皂苷合成的最高抑制率发生在接种后第3天，分别为57.94％和42.17％。通过对人参发根中单体皂苷Rg1、Re和Rb1含量的HPLC测定发现，洛伐他汀（50μmol/L ）对上述单体皂苷合成的最高抑制率依次为50.38％、66.66％和40.11％；膦胺霉素（15μmol/L ）对上述单体皂苷的最高抑制率依次为38.53％、38.85％和19.46％，与抑制剂对人参总皂苷含量的影响规律基本一致。推断人参皂苷的生物合成可能同时存在MVA和MEP途径，而MVA途径在人参皂苷的生物合成中可能起主导作用。
통과MVA여MEP도경적억제제락벌타정화련알매소불동농도급작용시간대인삼발근생장영향적연구，학정락벌타정화련알매소대인삼총조감합성적최고억제솔발생재접충후제3천，분별위57.94％화42.17％。통과대인삼발근중단체조감Rg1、Re화Rb1함량적HPLC측정발현，락벌타정（50μmol/L ）대상술단체조감합성적최고억제솔의차위50.38％、66.66％화40.11％；련알매소（15μmol/L ）대상술단체조감적최고억제솔의차위38.53％、38.85％화19.46％，여억제제대인삼총조감함량적영향규률기본일치。추단인삼조감적생물합성가능동시존재MVA화MEP도경，이MVA도경재인삼조감적생물합성중가능기주도작용。
In different concentration and feeding time of inhibitor for ginseng hairy root growth study ,we found that lovastatin and fosmidomycin did not inhibit the growth of hairy root when concentrations were lower than 50μmol/L and 15μmol/L ,and the most suitable feeding time was 3 days .Under the above-mentioned conditions ,the maximum inhibition rate of lovastatin and fosmidomycin on the biosynthesis of total saponin were 57.94% and 42.17% respectively .Experimental investigation on content of monomer saponin Rg1、Re and Rb1 in ginseng hairy root indi-cated that the maximum inhibition rate of lovastatin on Rg1、Re and Rb1 were 50.38% ,66.66% ,40.11% respectively ,and the maximum inhi-bition rate of fosmidomycin were 38.53% ,38.85% ,19.46% respectively ,which coincided with the results of variation of total saponin .There-fore ,we concluded that both MVA pathway and MEP pathway existed in the biosynthesis of ginsenosides ,and among them MVA pathway played the dominant role .